| 供货周期： | 一周 |
| 品牌： | |
| 规格： | 1mg/5mg/50mg |
| 货号： | ENZ-289 |
| CAS号： |
iCDH
Isocitrate Dehydrogenase (NADP +) Recombinant
Catalog No. ENZ-289
Synonyms:
Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.
Description:
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
The ICDH is purified by proprietary chromatographic techniques.
Source:
Saccharomyces Cerevisiae
Physical Appearance:
Sterile Filtered clear solution.
Formulation:
One ml of solution (1mg/84µl) contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
Stability:
ICDH although stable at 15°C for 1 week should be stored between
2°C-8°C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA)
Please avoid freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity:
The specific activity was found to be 30 IU/mg.
Unit Definition:
One unit is defined as 1 μmol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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