重组乳酸脱氢酶B(LDHB)

重组乳酸脱氢酶B(LDHB)

参考价：¥800
供货周期： 一周
品牌： Prospec-Tany
规格： 1mg
货号： ENZ-279
CAS号：
上海高创化学科技有限公司
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Synonyms Lactate Dehydrogenase, LDH.
Introduction Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.
A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Description The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.
Source Escherichia Coli.
Physical Appearance Sterile lyophilized powder.
Formulation The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Solubility It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity The specific activity was found to be 258 U/mg protein.
Unit Definition One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25°C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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