供货周期: | 一周 |
品牌: | |
规格: | 250U/750U/2500U |
货号: | ENZ-309 |
CAS号: |
Taq Plus DNA
Taq Plus DNA Polymerase Recombinant
Catalog No. ENZ-309
Synonyms:
Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.
Description:
Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78°C and remains > 95% active following 1-hour incubation at 95°C.
Source:
Recombinant E.coli contains Thermus aquaticus polymerase gene.
Unit Definition:
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74°C.
Storage Buffer:
20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.
Concentration:
5U/µl
10X Reaction Buffer:
200 mM TrisHCI (pH 8.8)
100 mM KCI
100 mM (NH4)2SO4
20 mM Mg SO4
1% Triton X-100
1 mg/ml bovine serum albumin (BSA).
Note: All reagents, including Taq Plus, should be mixed immediately before use.
Reaction Conditions:
DNA synthesis is performed in 100μl of mixture containing 20-200μM dNTPs, 0.3-1μM Promers, 0.1-0.250 mg of template DNA, 10 μl of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95º for 5 minutes and then cool to 40-68º for 5 minutes to allow the primers to anneal to the template DNA.
Optimization of DNA Synthesis:
It is important to ad the reaction components in the following order:
1. H2O
2. 10 x reaction buffer
3. dNTPs
4. DNA template and primers
5. Taq Plus
Storage Conditions:
Stable for 5 days at 10°C, for longer period of time store at -20°C.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
相关产品
重组人肌球蛋白轻链2(Recombinant Human MYL2)
重组大鼠抵抗素(Rat Resistin)
大鼠酸性磷酸酶(ACP)ELISA Kit
大鼠胆囊收缩素/缩胆囊素八肽(CCK-8)ELISA Kit
人瘦素(LEP)ELISA Kit
重组人半乳糖凝集素16/LGALS16 Human
重组人半乳糖凝集素8/Human LGALS8 His
重组人半乳糖凝集素3/Human LGALS3 His
重组人卵泡抑素/Human FST His
重组人β防御素118/DEFB118 Human
重组人心肌营养素1/CTF1 Human
特级新生牛血清(无支原体)
超级新生牛血清(无支原体)
优级胎牛血清(无支原体)
特级新生牛血清(无噬菌体低内毒素)
详细地址
关注
拨打电话
留言咨询