供货周期: | 一周 |
品牌: | |
规格: | 5μg/20μg/1mg |
货号: | PRO-390 |
CAS号: |
CENPB Human
Centromere Protein B Human Recombinant
Catalog No. PRO-390
Synonyms:
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.
Introduction:
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.
CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.
Description:
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.
CENPB is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
Source:
Sf9 insect cells.
Physical Appearance:
Sterile Filtered liquid formulation.
Formulation:
CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.
Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration:
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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