供货周期: | 一周 |
品牌: | Prospec-Tany |
规格: | 25ug |
货号: | cyt-120 |
CAS号: |
Synonyms | Fibroblast growth factor 18, FGF-18, zFGF5, FGF18. |
Introduction | Fibroblast growth factor 18 (FGF18) is a member of the large FGF family which has at least 23 members. FGF18 is a heparin binding growth factor with a core 120 amino acid FGF domain which allows for a common tertiary structure. FGFs are expressed in the course of the embryonic development and in restricted adult tissues. FGF-18 is an indispensable regulator of long bone and calvarial development. FGF-18 signals via FGFR 1c, 2c, 3c, and 4. |
Description | FGF-18 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 20.1kDa. The FGF-18 is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | FGF-18 protein was lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Solubility | It is recommended to reconstitute the lyophilized FGF-18 in sterile 18M?-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized FGF-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Amino Acid Sequence | AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSR. |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5ng/ml, corresponding to a specific activity of > 2.0×106 units/mg. |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Related Products | ? FGF 18 Mouse ? FGF-18 Rat |
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