产品详情
产品名称:人聚集蛋白(Agrin)ELISA Kit
英文名称:Human Agrin ELISA Kit
产品类型: ELISA Kit 别名: RP11-54O7.13, FLJ45064, agrin proteoglycan
种属: Human
待测物名称: agrin
缩写: AGRN
检测范围: Request Information
灵敏度: Request Information
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
用途: For research use only. Not for diagnostic use.
原理:This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any A......+
特异性:This assay has high sensitivity and excellent specificity for detection of Human AGRN. No significant cross-reactivity or interference between Human AGRN and analogues was observed.
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存:Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+
检测步骤:Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1.......+
结果计算:Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract ......+