供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-EL001490MO |
CAS号: |
产品类型:ELISA Kit
产品名称:小鼠同种异体移植炎症因子1(AIF1) ELISA kit
英文名称:Mouse Allograft inflammatory factor 1(AIF1) ELISA kit
别名: DADB-70P7.8, AIF-1, IBA1, IRT-1, interferon gamma responsive transcript|ionized calcium-binding adapter molecule
种属: Mouse
待测物名称: allograft inflammatory factor 1
缩写: AIF1
检测范围: 23.5 pg/ml-1500 pg/ml
灵敏度: 5.8 pg/ml
反应时间: 1-5h
所需样本体积: 50-100ul
检测波长: 450 nm
实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗同种异体移植炎症因子1(AIF1)抗体的微孔中依次加入标本或标准品、生物素化的同种异体移植炎症因子1(AIF1)抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的同种异体移植炎症因子1(AIF1)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔)。
2. 标准品(Standard):2瓶(冻干品)。
3. 样品稀释液(Sample Diluent):1×20ml/瓶。
4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶。
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。
人同种异体移植炎症因子1(AIF1) ELISA kit免费代测。
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