供货周期: | 一周 |
品牌: | Cusabio |
规格: | 96T |
货号: | CSB-EQ027323HU |
CAS号: |
人β2转铁蛋白(β2TF) ELISA kit
产品类型:ELISA Kit产品名称:人β2转铁蛋白(β2TF) ELISA kit
英文名称:Human beta 2 transferrin (β2TF) ELISA kit
种属: Human
待测物名称: beta 2 transferrin (β2TF)
缩写: β2TF
检测范围: 1.25 ug/ml-80 ug/ml
灵敏度: 0.31 ug/ml
反应时间: 1-5h
实验原理
用纯化的抗体包被微孔板,制成固相载体,往包被抗β2转铁蛋白(β2TF)抗体的微孔中依次加入标本或标准品、生物素化的β2转铁蛋白(β2TF)抗体、HRP标记的亲和素,经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的β2转铁蛋白(β2TF)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样品浓度。
试剂盒组成及试剂配制
1. 酶联板(Assay plate ):一块(96孔)。
2. 标准品(Standard):2瓶(冻干品)。
3. 样品稀释液(Sample Diluent):1×20ml/瓶。
4. 生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。
5. 辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
6. 生物素标记抗体(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根过氧化物酶标记亲和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶。
9. 浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。
10. 终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。
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