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Why use FocusClearTM Fluorescence labeling is essential for fluorescence microscopy and confocal microscopic analysis of biological samples. Since many fluorophores are quenched or extracted during routine dehydration procedures, most fluorescence-labeled samples are directly mounted in glycerol-based mounting media for fluorescence and confocal imaging. Under such conditions, a biological structure can be viewed at about 100μm beneath the surface using laser source with wavelength at visible spectrum range. To increase depth of view, one can use two-photon confocal system with infrared laser as light source and viewing structures twice deeper than the conventional confocal microscope. FocusClearTM is a water-soluble clearing agent for increases the transparency of biological tissues.
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