供货周期: | 7天 |
品牌: | |
型号: | 2μg |
货号: | CYT-722 |
Recombinant Dog Interleukin-3犬白介素3
Synonyms | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. | ||||||||
Introduction | IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. | ||||||||
Description | Interleukin-3 Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 14 kDa. The IL-3 is purified by proprietary chromatographic techniques. | ||||||||
Source | Escherichia Coli. | ||||||||
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | ||||||||
Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing 1X PBS pH7.4. | ||||||||
Solubility | It is recommended to reconstitute the lyophilized IL3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||||||
Stability | Lyophilized IL3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. | ||||||||
Purity | Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE. | ||||||||
Amino acid sequence | RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP. | ||||||||
Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. | ||||||||
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||||||
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