供货周期: | 一周 |
品牌: | |
规格: | 2μg |
货号: | PRO-421 |
CAS号: |
Synonyms | Regenerating islet-derived protein 3 alpha, Reg III-alpha, Pancreatitis-associated protein 1, REG3A, HIP, PAP, PAP1, REG3, INGAP, PAP-H, PBCGF, REG-III. | ||||
Introduction |
Pancreatitis-associated protein (PAP) is a secretory protein not normally expressed in healthy pancreas but highly induced during acute pancreatitis. While PAP has been shown to be anti-bacterial and antiapoptotic in vitro, its definitive biological function in vivo is not clear. Using antisepse oligonucleotides, inhibition of PAP expression significantly worsened pancreatitis in a rat model. During pancreatitis, PAP released by the pancreas could mediate lung inflammation through induction of hepatic TNF- alpha expression and subsequent increase in circulating TNF-alpha. PAP is activated in primary liver cancers. In normal liver, the protein is undetectable in normal mature hepatocytes and found only in some ductular cells, representing potential hepatic progenitor cells. PAP can be considered hepatic cytokine that combines mitogenic and anti-apoptotic functions regarding hepatocytes, and consequently acts as a growth factor in vivo to enhance liver regeneration. In pancreatic cancor, PAP was overexpressed in 79% (30 of 38) of pancreatic ductal adenocarcinoma, 19% (7 of 36) of chronic pancreatitis, and 29% (2 of 7) of mucinous cystadenoma. PAP was found in malignant ductular structures in pancreatic carcinomas as well as in benign proliferating ductules and acinar cells in chronic pancreatitis. Elevation of PAP in patients with pancreatic cancer is not merely explainable by concomitant pancreatitis, but seems to be due to increased PAP production by the cancer cells and is also correlated to tumour load as expressed by the UICC stages. Epithelial expression of PAP was induced under intestinal mucosal inflammation initiated by exposure to commensal bacteria or DSS as well as inflamed IBD colon. Increased serum level of PAP diagnosed ileal location in active Crohn disease with a sensitivity of 60%, a specificity of 94%, a positive predictive value of 84% and a negative predictive value of 81%. Elevated serum PAP (> 50 ng/mL) is significantly associated with disease activity and ileal location of Crohn disease. |
||||
Description | The Recombinant Human REG3A protein is produced with N-terminal fusion of His Tag. The REG3A His -Tagged Fusion Protein is an 18.4 kDa protein containing 149 amino acid residues of the Human REG3A and 16 additional amino acid residues- His Tag (underlined). | ||||
Source | Escherichia Coli. | ||||
Physical Appearance | Filtered White lyophilized (freeze-dried) powder. | ||||
Formulation | Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. | ||||
Purity | Greater than 95.0% as determined by SDS-PAGE. | ||||
Solubility | It is recommended to add 0.1M Acetate buffer pH-4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. | ||||
Stability | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. | ||||
Amino Acid Sequence | MRGSHHHHHH GMASHMEEPQ RELPSARIRC PKGSKAYGSH CYALFLSPKS WTDADLACQK RPSGNLVSVL SGAEGSFVSS LVKSIGNSYS YVWIGLHDPT QGTEPNGEGW EWSSSDVMNY FAWERNPSTI SSPGHCASLS RSTAFLRWKD YNCNVRLPYV CKFTD. | ||||
Applications | Western blotting, ELISA. | ||||
Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
Related Products |
|
相关产品
猴糜蛋白酶样弹性蛋白酶1(ELA1)ELISA kit
猴弹性蛋白酶2,中性粒细胞(ELA2)ELISA kit
牛脂多糖/内毒素(LPS) ELISA kit
大鼠细胞色素P450 2B1(Cyp2b1)ELISA kit
Dog Cytochrome P450 2B11(Cyp2b11) ELISA kit
Mouse Methylthioadenosine Phosphorylase(MTAP)ELISA kit
豚鼠胰淀粉酶α2A(AMY2A)ELISA Kit
猪胰岛素样生长因子1(IGF-1)ELISA Kit
人Klotho ELISA Kit
小鼠Klotho ELISA Kit
小鼠嗅觉标记蛋白(OMP)ELISA kit
人运甲状腺素蛋白/前白蛋白(TTR)ELISA kit
Human Placenta-specific gene 8 protein(PLAC8) ELISA kit
小鼠L氨基酸氧化酶(IL4I1)ELISA kit
植物过氧化氢酶2(CAT2/CAT)ELISA kit
Recombinant Human Beta-Site APP-Cleaving Enzyme 1
面议
Recombinant Human CD11b
面议
Recombinant Human CD14 CHO derived
面议
Recombinant Human Chromosome 19 Open Reading Frame 10
面议
Recombinant Human D4-GDI
面议
Recombinant Human ELK-1
面议
Recombinant Human Homer Homolog-1
面议
Recombinant Human Mothers Against Decapentaplegic Homolog 3
面议
详细地址
关注
拨打电话
留言咨询