RNA沉默在分析植物基因功能方面的研究

2016-01-21 07:51  下载量:1

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The research on the analysis of gene function in plants mediated by RNA silencing NIU Yan-Bing*, GUO Shi-Mi, SHEN Lin-Yan, LEI Wan-Jun, LEI Xiao-Fei (Shanxi Agricultural University, Taigu 030801, China) Abstract: Being a highly conserved and sequence-specific RNA degradation system in eukaryotic, RNA silencing is both a fundamental biological issue and a highly promising application upon the control of gene expression or the analysis of gene function. Posttranscriptional gene silencing (PTGS) in plants is a form of RNA silencing by which target RNA is degraded in a sequence-specific manner. Realizing regarding the central role that double-stranded RNA plays in triggering RNA silencing has prompted the development of several vectors construction for RNA silencing. These methods, in combination with the genomic resources, by means of transgenic and non-transgenic technology, have provided rapid and efficient means by which to investigate gene function in a wide range of plant species. Key words: RNA silencing ; analysis; GENE function rna沉默是真核生物的一种高度保守的和序列特异的RNA降解系统,该系统包括植物中的转录后基因沉默(PTGS)、动物中的RNA 干扰(RNAi)和真菌中的消除作用(quelling)

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