1)包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
Y165497 MHC(Human myosin heavy chain) ELISA Kit 人肌球蛋白重链 48T
Y165498 cTnT(Human cardiac isoform of Tropnin T) ELISA Kit 人心肌特异性肌钙蛋白T 48T
Y165499 SMM(Human smooth muscle Myosin) ELISA Kit 人平滑肌肌球蛋白 48T
Y165500 Alpha-SCA(Human Alpha-sarcomeric actin) ELISA Kit 人α横纹肌肌动蛋白 48T
Y165501 Alpha-SMA(Human Alpha-Smooth muscle actin) ELISA Kit 人α平滑肌肌动蛋白 48T
Y165502 sVE-Cadherin (Human Vascular Endothelial-Cadherin Complex) ELISA Kit 人血管内皮钙粘着蛋白复合体 48T
Y165503 8-epi-PGF2 Alpha(Human 8-epi-prostaglandin F2alpha0 ELISA Kit 人8-异构前列腺素F2α 48T
Y165504 IMA(Human Ischemia Modified Albumin) ELISA Kit 人缺血修饰白蛋白 48T
Y165505 CMLC-1(Human Cardiac myosin-light chains 1) ELISA Kit 人心肌肌凝蛋白轻链1 48T
Y165506 VPI(Human vasopeptidase inhibitors) ELISA Kit 人血管活性肽酶抑制剂 48T
Y165507 HSF-1(Human Heat Shock Factor 1) ELISA Kit 人热休克因子1 48T
Y165508 sMHC-1(Human soluble myosin heavy chain 1) ELISA Kit 人可溶性肌球蛋白重链1 48T
Y165509 ANG-4(Human Angiopoietin 4) ELISA Kit 人血管生成素4 48T
Y165510 ANG II R-1(Human angiotension II receptor 1) ELISA Kit 人血管紧张素Ⅱ受体1 48T
Y165511 AT2R(Human Angiotensin II Receptor 2) ELISA Kit 人血管紧张素Ⅱ受体2 48T
Y165512 LEPR(Human Leptin Receptor) ELISA Kit 人瘦素受体 48T
Y165513 ANKRD1(Human cardiac ankyrin repeat domain 1) ELISA Kit 人心肌锚蛋白重复域1 48T
Y165514 Alpha Actin(Human Alpha-Actin) ELISA Kit 人α肌动蛋白 48T
Y165515 ANGPTL3(Human angiopoietin-like protein 3) ELISA Kit 人血管生成素样蛋白3 48T
Y165516 Agtr I a(Human angiotensin II receptor type I a) ELISA Kit 人血管紧张素Ⅱ受体Ⅰa型 48T
Y165517 ES-Ab(Human anti-Endostatin antibody) ELISA Kit 人血管内皮抑素抗体 48T
Y165518 Tn-T(Human Troponin T) ELISA Kit 人肌钙蛋白T 48T
Y165519 AP36(Human Apelin 36) ELISA Kit 人Apelin 36 48T
Y165520 AP13(Human apelin 13) ELISA Kit 人apelin 13 48T
Y165521 TBM(Human tubular basement membrane antibogy) ELISA Kit 人抗肾小管基底膜抗体 48T
Y165522 ANX- V (Human Annexin V ) ELISA Kit 人膜联蛋白Ⅴ 48T
Y165523 BMG/ Beta2-MG(Human Beta2-microglobulin) ELISA Kit 人β2微球蛋白 48T
Y165524 GBM(Human glomerular basement membrane antibogy) ELISA Kit 人抗肾小球基底膜抗体 48T
Y165525 ACA(Human adrenal cortex antibody) ELISA Kit 人肾上腺皮质抗体 48T
Y165526 ASO(Human anti-streptolysin O) ELISA Kit 人抗链球菌溶血素O/抗O 48T
Y165527 RF(Human rheumatoid factor) ELISA Kit 人类风湿因子 48T
Y165528 AQP-0(Human Aquaporin 0) ELISA Kit 人水通道蛋白0 48T
Y165529 AQP-1(Human Aquaporin 1) ELISA Kit 人水通道蛋白1 48T
Y165530 AQP-2(Human Aquaporin 2) ELISA Kit 人水通道蛋白2 48T
Y165531 AQP-3(Human Aquaporin 3) ELISA Kit 人水通道蛋白3 48T
Y165532 AQP-4(Human Aquaporin 4) ELISA Kit 人水通道蛋白4 48T
Y165533 AQP-5(Human Aquaporin 5) ELISA Kit 人水通道蛋白5 48T
Y165534 Kim-1(Human Kidney injury molecule 1) ELISA Kit 人肾损伤分子1 48T
Y165535 Alpha2-MG(Human Alpha2 macroglobulin) ELISA Kit 人α2巨球蛋白 48T
Y165536 LMP7/PSMB99Human low molecular-weight protein/proteasome beta-type subunit) ELISA Kit 人低分子质量蛋白7/β型蛋白酶体9 48T
Y165537 UP(Human Proteinuria) ELISA Kit 人尿蛋白 48T
Y165538 GTE-AGE(Human Glomerular tissue extract-advanced glycosylation end products) ELISA Kit 人肾小球组织糖基化终末产物 48T
Y165539 THP(Human T-H glycoprotein) ELISA Kit 人TH糖蛋白 48T
Y165540 RANA(Human rheumatoid arthritis associated nuclear artigen) ELISA Kit 人抗类风湿关节炎核抗原 48T
Y165541 AAA(Human Anti-adrenocortical antibody) ELISA Kit 人抗肾上腺皮质抗体 48T
Y165542 ALB(Human microalbunminuria) ELISA Kit 人尿微量白蛋白 48T
Y165543 Alpha1-MG(Human Alpha1-microglobulin) ELISA Kit 人α1微球蛋白 48T
Y165544 Human Nephrin ELISA Kit 人肾病蛋白 48T
Y165545 Human podocin ELISA Kit 人Podocin 48T
Y165546 AMBP(Human alpha-1-microglobulin/bikunin precursor) ELISA Kit 人α1微球蛋白/bikunin前体 48T
Y165547 ACE(Human Angiotensin converting enzyme) ELISA Kit 人血管紧张素转化酶 48T
Y165548 Flt-3L(Human FMS-like tyrosine kinase 3 ligand) ELISA Kit 人FMS样酪氨酸激酶3配体 48T
Y165549 Flt3(Human FMS-like tyrosine kinase 3) ELISA Kit 人FMS样酪氨酸激酶3 48T
Y165550 MMP-1(Human Matrix metalloproteinase 1) ELISA kit 人基质金属蛋白酶1 48T
Y165551 MMP-10(Human Matrix metalloproteinase 10) ELISA kit 人基质金属蛋白酶10 48T
Y165552 MMP-13(Human Matrix metalloproteinase 13) ELISA kit 人基质金属蛋白酶13 48T
Y165553 MMP-2/Gelatinase A(Human Matrix metalloproteinase 2/Gelatinase A) ELISA kit 人基质金属蛋白酶2/明胶酶A 48T
Y165554 MMP-3(Human Matrix metalloproteinase 3) ELISA kit 人基质金属蛋白酶3 48T
Y165555 (MMP-7(Human Matrix metalloproteinase 7) ELISA kit 人基质金属蛋白酶7 48T
Y165556 MMP-8(Human Matrix metalloproteinase 8/Neutrophil collagenase) ELISA kit 人基质金属蛋白酶8/中性粒细胞胶原酶 48T
Y165557 Tie-2(Human tyrosine kinase with immunoglobulin-like and EGF-like domains 2 ELISA Kit 人血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶2 48T
Y165558 uPA(Human urokinase plasminogen activator) ELISA kit 人尿激酶型纤溶酶原激活物 48T
Y165559 PLAUR/uPAR(Human Urokinase-type Plasminogen Activator Receptor) ELISA kit 人尿激酶型纤溶酶原激活物受体 48T
Y165560 PG-A(Human Pepsinogen A) ELISA Kit 人胃蛋白酶原A 48T
Y165561 PG-C(Human Pepsinogen C) ELISA Kit 人胃蛋白酶原C 48T
Y165562 PGDS(Human Prostaglandin D Synthase) ELISA Kit 人前列腺素D合成酶 48T
Y165563 PAP(Human Prostatic Acid Phosphatase) ELISA Kit 人前列腺酸性磷酸酶 48T
Y165564 CK-MB(Human Creatine Kinase MB isoenzyme) ELISA Kit 人肌酸激酶同工酶MB 48T
Y165565 NSE(Human Neuron-specific enolase) ELISA Kit 人神经特异性烯醇化酶 48T
Y165566 MMP-9/Gelatinase B(Human matrix metalloproteinase 9/Gelatinase B) ELISA Kit 人基质金属蛋白酶9/明胶酶B 48T
Y165567 MMP-4(Human matrix metalloproteinase 4) ELISA Kit 人基质金属蛋白酶4 48T
Y165568 MMP-5(Human matrix metalloproteinase 5) ELISA Kit 人基质金属蛋白酶5 48T
Y165569 TE(Human telomerase) ELISA Kit 人端粒酶 48T
Y165570 NOS(Human nitric oxide synthase) ELISA Kit 人一氧化氮合成酶 48T
Y165571 PL-A2(Human Phospholipidase A2) ELISA Kit 人磷脂酶A2 48T
Y165572 iNOS(Human inducible nitric oxide synthase) ELISA KIT 人诱导型一氧化氮合成酶 48T
Y165573 HO-1(Human heme oxygenase 1) ELISA Kit 人血红素氧合酶1 48T
Y165574 eNOS(Human Endothelial nitric oxide synthase) ELISA Kit 人内皮型一氧化氮合成酶 48T
Y165575 AFU(Human alpha-L-fucosidase) ELISA Kit 人αL岩藻糖苷酶 48T
Y165576 TPO(Human Thyroid-Peroxidase) ELISA Kit 人甲状腺过氧化物酶 48T
Y165577 M2-PK (Human Pyruvate Kinase) ELISA Kit 人丙酮酸激酶M2型同工酶 48T
Y165578 HO-2(Human heme oxygenase 2) ELISA Kit 人血红素氧合酶2 48T
Y165579 PKB(Human protein kinase B) ELISA Kit 人蛋白激酶B 48T
Y165580 PI3K(Human Phosphotylinosital 3 kinase) ELISA Kit 人磷酸肌醇3激酶 48T
Y165581 TRACP-5b9Human tartrate-resistant acid phosphatase 5b) ELISA Kit 人抗酒石酸酸性磷酸酶5b 48T
Y165582 Human Methylase ELISA Kit 人甲基化酶 48T
Y165583 HD(Human Histone Deacetylase) ELISA Kit 人组织蛋白去乙酰化酶 48T
Y165584 DPP IV (Human dipeptidyl peptldase IV ) ELISA Kit 人二肽基肽酶Ⅳ 48T
Y165585 SOD(Human Super Oxidase Dimutase) ELISA Kit 人超氧化物歧化酶 48T
Y165586 CIAP(Human Calf intestinal alkaline phosphatase) ELISA Kit 人牛小肠碱性磷酸酶 48T
Y165587 Gzms-A(Human granzymes A) ELISA Kit 人颗粒酶A 48T
Y165588 Gzms-B(Human granzymes B) ELISA Kit 人颗粒酶B 48T
Y165589 MPO(Human myeloperoxidase) ELISA Kit 人髓过氧化物酶 48T
Y165590 ADAM8(Human A Disintegrin And Metalloprotease 8) ELISA Kit 人解整合素样金属蛋白酶8 48T
Y165591 CA-2(Human carbonic anhydrase 2) ELISA Kit 人碳酸酐酶2 48T
Y165592 牛β防御素(β-Defensin)ELISA KitNE(Human neutrophil elastase) ELISA Kit 人中性粒细胞弹性蛋白酶 48T
Y165593 Casp-3(Human Caspase 3) ELISA Kit 人胱天蛋白酶3 48T
Y165594 Casp-9(Human Caspase-9) ELISA Kit 人胱天蛋白酶9 48T
Y165595 GSK(Human glycogen synthase kinase) ELISA Kit 人糖原合成酶激酶 48T
Y165596 PDH E1(Human pyruvate dehydrogenase-E1) ELISA Kit 人丙酮酸脱氢酶E1 48T
Y165597 HK(Human Hexokinase) ELISA Kit 人己糖激酶 48T
Y165598 PP(Human Pepsin) ELISA Kit 人胃蛋白酶 48T