1)包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
Y165812 CETP(Human glial fibrillary acidic protein) ELISA Kit 人胆固醇酯转移蛋白 48T
Y165813 15-LO/LOX(Human 15-lipoxygenase) ELISA Kit 人15脂加氧酶 48T
Y165814 GFAP(Human glial fibrillary acidic protein) ELISA Kit 人神经胶质纤维酸性蛋白 48T
Y165815 CsA(Human Cyclosporine A) ELISA Kit 人环孢素A 48T
Y165816 PPAR- Gamma(Human Peroxisome Proliferator-activated receptor Gamma) ELISA Kit 人过氧化物酶体增殖因子活化受体γ 48T
Y165817 ET(Human endotoxin) ELISA Kit 人内毒素 48T
Y165818 FA(Human Folic acid) ELISA Kit 人叶酸 96T
Y165819 ON(Human osteonectin) ELISA Kit 人骨粘连蛋白 48T
Y165820 FFA(Human free fatty acids) ELISA Kit 人游离脂肪酸 48T
Y165821 Hcy(Human Homocysteic acid) ELISA Kit 人同型半胱氨酸 48T
Y165822 GSH-Px kit(human) 人谷胱甘肽过氧化物酶elisa试剂盒 96T
Y165823 Alpha-GST(Human Alpha-glutathione S-transferases) ELISA Kit 人α谷胱甘肽S转移酶 48T
Y165824 HDL(Human High density lipoprotein) ELISA Kit 人高密度脂蛋白 48T
Y165825 LDLR(Human very low density lipoprotein receptor) ELISA Kit 人低密度脂蛋白受体 48T
Y165826 VLDLR(Human very low density lipoprotein receptor) ELISA Kit 人极低密度脂蛋白受体 48T
Y165827 LRP-6(Human low-density lipoprotein-receptor-related protein) ELISA Kit 人低密度脂蛋白受体相关蛋白6 48T
Y165828 N-MID-OT(Human N-MID Osteocalcin) ELISA Kit 人N端中段骨钙素 48T
Y165829 HDL-C(Human High density lipoprotein cholesterol) ELISA Kit 人高密度脂蛋白胆固醇 48T
Y165830 Beta-OHB(Human Beta-Hydroxybutyric Acid) ELISA Kit 人β羟丁酸 48T
Y165831 1,5-AG(Human 1,5-anhydroglucitol) ELISA Kit 人1,5-脱水葡萄糖醇/1,5-脱水山梨 48T
Y165832 1,3-DPG(Human 1,3-Disphosphoglycerate, ELISA Kit 人1,3-二磷酸甘油酸 48T
Y165833 CTX(Human Cyclophosphamide) ELISA Kit 人环磷酰胺 48T
Y165834 BALP(Human bone alkaline phosphatase) ELISA Kit 人骨碱性磷酸酶 48T
Y165835 BSP(Human bone sialoprotein) ELISA Kit 人骨涎蛋白 48T
Y165836 AA(Human Arachidonic Acid) ELISA Kit 人花生四烯酸 48T
Y165837 LDL(Human low density lipoprotein) ELISA Kit 人低密度脂蛋白 48T
Y165838 GLUT1(Human Glucose transporter 1) ELISA Kit 人葡萄糖转运蛋白1 48T
Y165839 HRGP(Human Hydroxyproline-Rich Glycoprotein) ELISA Kit 人羟脯氨酸糖蛋白 48T
Y165840 ABCA1(Human ATP-binding cassette transporter A1) ELISA Kit 人腺苷三磷酸结合盒转运体A1 48T
Y165841 FABP(Human fatty acid-binding protein) ELISA Kit 人脂肪酸结合蛋白 48T
Y165842 BMP-6(Human Bone Morphogenetic Protein 6) ELISA Kit 人骨形成蛋白6 48T
Y165843 CR(Human Calretinin) ELISA Kit 人钙结合蛋白 48T
Y165844 Human CTX-2 ELISA Kit 人骨退化特异标志物 48T
Y165845 CaN(Human calcineurin) ELISA Kit 人钙调磷酸酶 48T
Y165846 CALD(Human Caldesmon) ELISA Kit 人钙调结合蛋白 48T
Y165847 ADRP(Human adipose differentiation-related protein) ELISA Kit 人脂肪分化相关蛋白 48T
Y165848 HA(Human hemagglutinin) ELISA Kit 人血凝素 48T
Y165849 GKRP(Human glucokinase regulatory protein) ELISA Kit 人葡萄糖激酶调节蛋白 48T
Y165850 VMA(Human vanillylmandelic acid) ELISA Kit 人垂草扁桃酸 48T
Y165851 PLP(Human proteolipid protein) ELISA Kit 人蛋白脂质蛋白抗体 48T
Y165852 RBP-4(Human Retinol binding protein 4) ELISA Kit 人视黄醇结合蛋白4 48T
Y165853 BMPs(Human bone morphogenetic proteins) ELISA Kit 人骨形成蛋白 48T
Y165854 cath-K(Human Cathepsin K) ELISA Kit 人组织蛋白酶K 48T
Y165855 17-OHCS(Human 17-Hydroxycorticosteroids) ELISA Kit 人17羟皮质类固醇 48T
Y165856 17-KS(Human 17-ketosteroids) ELISA Kit 人17-酮类固醇 48T
Y165857 GSH(Human glutathione) ELISA Kit 人谷胱甘肽 48T
Y165858 GSH-Px(Human Glutathione peroxidase) ELISA Kit 人谷胱甘肽过氧化酶 48T
Y165859 LBP(Human lipolysaccharide binding protein) ELISA Kit 人脂多糖结合蛋白 48T
Y165860 FEP(Human Free erythrocyte proloporphyrin) ELISA Kit 人游离原卟啉 48T
Y165861 ConA(Human concanavalin A) ELISA Kit 人刀豆素A 48T
Y165862 SPA(Human Staphylococal protein A) ELISA Kit 人葡萄球菌蛋白A 48T
Y165863 PYD(Human Pyridinoline crosslinks) ELISA Kit 人胶原吡啶交联 48T
Y165864 DPD(Human Deoxypyridinoline crosslinks) ELISA Kit 人脱氧胶原吡啶交联 48T
Y165865 OH-PYD(Human Hydroxy-Pyridinium crosslinks) ELISA Kit 人羟基胶原吡啶交联 48T
Y165866 ODF(Human osteoclast differentiation factor) ELISA Kit 人破骨细胞分化因子 48T
Y165867 OGP(Human Osteogenic Growth Peptide) ELISA Kit 人成骨生长肽 48T
Y165868 Apo-E(Human Apolipoprotein E) ELISA Kit 人载脂蛋白E 48T
Y165869 PPAR- Alpha(Human peroxisome proliferators activator receptors alpha) ELISA Kit 人过氧化物酶体增殖物激活受体α 48T
Y165870 Human preptin ELISA Kit 人preptin 48T
Y165871 LPS(Human Lipopolysaccharides) ELISA Kit 人脂多糖/内毒素 48T
Y165872 PWM(Human pokeweed mitogen) ELISA Kit 人美洲商陆素 48T
Y165873 GPI(Human glycophosphatidylinositol) ELISA Kit 人糖磷脂酰肌醇 48T
Y165874 Apo-H(Human Apolipoprotein H) ELISA Kit 人载脂蛋白H 48T
Y165875 GAP(Human GTPase activating protein) ELISA Kit 人GTP酶活化蛋白 48T
Y165876 Hyl(Human hydroxylysine) ELISA Kit 人羟赖氨酸 48T
Y165877 8-OHdG(Human hydroxylysine) ELISA Kit 人8羟基脱氧鸟苷 48T
Y165878 RBP(Human Retinol binding protein) ELISA Kit 人视黄醇结合蛋白 48T
Y165879 GLUT3(Human Glucose transporter 3) ELISA Kit 人葡萄糖转运蛋白3 48T
Y165880 GPC-3(Human Glypican-3) ELISA Kit 人磷脂酰肌醇蛋白聚糖3 48T
Y165881 aFABP(Human adipocyte fatty acid-binding protein) ELISA Kit 人脂肪细胞型脂肪酸结合蛋白 48T
Y165882 LCN2(Human lipocalin 2) ELISA Kit 人脂质运载蛋白2 48T
Y165883 cytb561(Human lipocalin 2) ELISA Kit 人细胞色素b561 48T
Y165884 Gbp4(Human guanylate nucleotide binding protein 4) ELISA Kit 人鸟苷酸结合蛋白4 48T
Y165885 SAA3(Human serum amyloid A3) ELISA Kit 人血清淀粉样蛋白A3 48T
Y165886 SEP1(Human selenoprotein 1) ELISA Kit 人硒蛋白1 48T
Y165887 DBP(Human Vitamin D-binding protein) ELISA Kit 人维生素D结合蛋白 48T
Y165888 apo-A5(Human apoprotein A5) ELISA Kit 人载脂蛋白A5 48T
Y165889 CALU(Human calumenin) ELISA Kit 人钙腔蛋白 48T
Y165890 CysLTs(Human cysteinyl leukotrienes) ELISA Kit 人半胱氨酰白三烯 48T
Y165891 GAG(Human glycosaminoglycan) ELISA Kit 人糖胺聚糖 48T
Y165892 3-NT(Human 3-Nitrotyrosine) ELISA Kit 人3-硝基酪氨酸 48T
Y165893 sLOX-1(Human Soluble Lectin-like oxidized low density lipoprotein receptor-1) ELISA Kit 人可溶性凝集素样氧化低密度脂蛋白受体1 48T
Y165894 MT2(Human Metallothionein 2) ELISA Kit 人金属硫蛋白2 48T
Y165895 CH(Human cholesterol) ELISA Kit 人血清总胆固醇 48T
Y165896 Apo C3(Human Apolipoprotein C3) ELISA Kit 人载脂蛋白C3 48T
Y165897 TBA(Human Total bile acide) ELISA Kit 人总胆汁酸 48T
Y165898 PC/CPG(Human choline phosphoglyceride) ELISA Kit 人胆碱磷酸甘油酯 48T
Y165899 PS(Human phosphatidylserine) ELISA Kit 人磷脂酰丝氨酸 48T
Y165900 PSA(Human prostate specific antigen) ELISA Kit 人前列腺特异性抗原 48T
Y165901 fPSA(Human Free Prostate Specific Antigen) ELISA Kit 人游离前列腺特异性抗原 48T
Y165902 HSV-Ag2(Human Herpes simplex virus 2) ELISA Kit 人单纯疱疹病毒抗原2 48T
Y165903 HPV-IgG(Human papillomavirus IgG) ELISA Kit 人乳头状瘤病毒抗体IgG 48T
Y165904 HPV-IgM(Human papillomavirus IgM) ELISA Kit 人乳头状瘤病毒抗体IgM 48T
Y165905 Human Estrogen-induced protein pS2 ELISA Kit 人雌激素诱导蛋白PS2 48T
Y165906 HSV I Ab牛前列腺素E2(PGE2)ELISA Kit(Human Herpes simplexvirus I antibody) ELISA Kit 人单纯疱疹病毒Ⅰ型抗体 48T
Y165907 BV(Human bacterial vaginosis) ELISA Kit 人细菌性阴道病 48T