1)包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
Y166114 CT(Human Chlamydia trachomatis) ELISA Kit 人沙眼衣原体 48T
Y166115 AGAA(Human anti-golgi apparatus antibody) ELISA Kit 人抗高尔基体抗体 48T
Y166116 ACA-IgG(Human anti-cardiolipin antibody IgG) ELISA Kit 人抗心磷脂抗体IgG 48T
Y166117 ACA-IgM(Human anti-cardiolipin antibody IgM) ELISA Kit 人抗心磷脂抗体IgM 48T
Y166118 Beta2-GP1 Ab IgG(Human Beta2-Glycoprotein 1 Antibody IgG) ELISA Kit 人β2糖蛋白1抗体IgG 48T
Y166119 Beta2-GP1 Ab IgM(Human Beta2-Glycoprotein 1 Antibody IgM) ELISA Kit 人β2糖蛋白1抗体IgM 48T
Y166120 ACA-IgA(Human anti-cardiolipin antibody IgA) ELISA Kit 人抗心磷脂抗体IgA 48T
Y166121 Beta2-GP1 Ab IgA(Human Beta2-Glycoprotein 1 antibody IgA) ELISA Kit 人β2糖蛋白1抗体IgA 48T
Y166122 PF(Human Pemphigus Foliaceus) ELISA Kit 人落叶型天疱疮抗体 48T
Y166123 Human SS-B/La Antibody ELISA Kit 人抗SS-B/La抗体 48T
Y166124 SCL70/topo I (Human anti-scl70 antibody) ELISA Kit 人抗硬皮病抗体70 48T
Y166125 AnuA-IgG(Human anti-nucleosome antibody IgG) ELISA Kit 人抗核小体抗体IgG 48T
Y166126 dsDNA(Human anti-double stranded DNA) ELISA KIT 人抗双链DNA抗体/天然DNA抗体 48T
Y166127 Human histone H2A-H2B-DNA autoantibody ELISA Kit 人抗组蛋白(H2A-H2B)-DNA抗体/抗二聚体-DNA抗体 48T
Y166128 ssDNA(human single stranded DNA Antibody) ELISA Kit 人抗单链DNA抗体/变性DNA抗体 48T
Y166129 Alpha-Fodrin IgG/IgA(Human anti-alpha-Fodrin IgG/IgA) ELISA Kit 人抗α-胞衬蛋白抗体IgG/IgA 48T
Y166130 PR3 Ab-IgG(Human Anti-proteinase 3 antibody IgG) ELISA Kit 人抗蛋白酶3抗体IgG 48T
Y166131 MPO-ANCA IgG(Human Mouse myeloperoxidase-antineutrophil cytoplasmic antibody IgG) ELISA kit 人髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG 48T
Y166132 BPI-Ab(Human anti-Bactericidal permeability increasing protein antibody) ELISA Kit 人抗杀菌通透性增高蛋白抗体 48T
Y166133 LF/LTF Ab-Ig(Human Lactoferrin antibody IgG) ELISA kit 人乳铁传递蛋白/乳铁蛋白抗体IgG 48T
Y166134 Cath Ab(Human Cathepsin Antibodies) ELISA Kit 人组织蛋白酶抗体 48T
Y166135 Beta2-GP1 IgA/G/M(Human Beta2-glycoprotein 1 antibody IgA/G/M) ELISA Kit 人β2糖蛋白1抗体IgA/G/M 48T
Y166136 PI Ab-IgG/IgM(human phosphatidylinositol antibody IgG/IgM) ELISA Kit 人磷脂酰肌醇抗体IgG/IgM 48T
Y166137 aPT1/aPT2(Human anti-prothrombins antibodies) ELISA Kit 人抗凝血素抗体 48T
Y166138 cANCA(human anti-neutrophil cytoplasmic antibody) ELISA Kit 人抗中性粒细胞胞浆抗体 48T
Y166139 pANCA(Human Perinuclear anti-neutrophil cytoplasmic antibody) ELISA Kit 人抗中性粒细胞核周抗体 48T
Y166140 AKA(Human Anti-keratin antibody) ELISA Kit 人抗角蛋白抗体 48T
Y166141 MSA(Human mitotic spindle apparatus antibody) ELISA Kit 人抗纺锤体抗体 48T
Y166142 SDA(Human salivary duct autoantibody) ELISA Kit 人抗唾液腺导管组织抗体 48T
Y166143 ICA(Human islet cell antibody) ELISA Kit 人胰岛细胞抗体 48T
Y166144 TAb(Human Thyroid Antibody) ELISA Kit 人甲状腺抗体 48T
Y166145 ASA(Human Anti-Somatic Muscle Antibody) ELISA Kit 人抗骨骼肌抗体 48T
Y166146 ARPA/Rib-P(Human anti-ribosomal P protein antibody) ELISA Kit 人抗核糖体P蛋白抗体 48T
Y166147 AT(Human Anti-trypsin) ELISA Kit 人抗胰蛋白酶 48T
Y166148 ALG(Human anti-lymphocyte globulin) ELISA Kit 人抗淋巴细胞球蛋白 48T
Y166149 PA-IgG/M/A(Human platelet antibodies IgG/M/A) ELISA Kit 人抗血小板抗体IgG/M/A 48T
Y166150 GAD-Ab(Human glutamic acid decarboxylase autoantibody) ELISA Kit 人谷氨酸脱羧酶自身抗体 48T
Y166151 ATGA/TGAB(Human Anti-Thyroid Microsome Antibody) ELISA KIT 人抗甲状腺球蛋白抗体 48T
Y166152 ATMA/TMAB(Human Anti-Thyroid Microsome Antibody) ELISA KIT 人抗甲状腺微粒体抗体 48T
Y166153 AMA(Human Anti-myocardial antibody) ELISA Kit 人抗心肌抗体 48T
Y166154 Human anti-IVIgG antibody ELISA Kit 人抗IVIgG抗体 48T
Y166155 Human anti-IgE receptor antibody ELISA Kit 人抗IgE受体抗体 48T
Y166156 PCNA(Human proliferating cell nuclear antigen antibody) ELISA Kit 人抗增殖细胞核抗原抗体 48T
Y166157 Human LDL-IC ELISA Kit 人低密度脂蛋白免疫复合物 48T
Y166158 EJ/GlyRS(Human anti-EJ-antibody) ELISA Kit 人EJ抗体/抗甘氨酰tRNA合成酶抗体 48T
Y166159 OLAb(Human oxidized lowdensity lipoprotein antibody) ELISA Kit 人氧化低密度脂蛋白抗体 48T
Y166160 AMA(Human anti-Monocyte antibody) ELISA Kit 人抗单核细胞抗体 48T
Y166161 GP(Human anti-glycoprotein antibody) ELISA Kit 人抗糖蛋白抗体 48T
Y166162 EMA IgA(Human anti-Endomysial Antibody IgA) ELISA Kit 人抗肌内膜抗体IgA 48T
Y166163 Human Beta anti-galactan proteins IgG ELISA Kit 人β乳糖蛋白抗体IgG 48T
Y166164 SLA(Human Proteinase 3 Antibody) ELISA Kit 人抗肝可溶性抗原抗体 48T
Y166165 PR3Ab(Human Proteinase 3 Antibody) ELISA Kit 人蛋白酶3抗体 48T
Y166166 HIT(Human heparin associated antibody) ELISA Kit 人抗肝素PF4复合物抗体/HIT抗体 48T
Y166167 AAA(Human anti-Actin antibody) ELISA Kit 人抗肌动蛋白抗体 48T
Y166168 DNP-Ab(Human deoxyribonucleic protein antibody) ELISA Kit 人抗脱氧核糖核蛋白抗体 48T
Y166169 ENA-Ab(Human anti-extractable nuclear antigen antibody) ELISA Kit 人抗可提取核抗原抗体 48T
Y166170 n-DNA-Ab(Human natural deoxyribonucleic acid antibody) ELISA Kit 人抗天然脱氧核糖核酸抗体 48T
Y166171 RNP-Ab(Human anti-ribonucleo protein Antibody) ELISA Kit 人抗核糖核蛋白抗体 48T
Y166172 Jo1/HRS(Human Jo-1 Antibody) ELISA Kit 人Jo1抗体/抗组氨酰tRNA合成酶抗体 48T
Y166173 Sc1-70-Ab(Human Sc1-70 Antibody) ELISA Kit 人抗Sc1-70抗体 48T
Y166174 IFN Alpha-Ab(Human Interferon AlphaAntibody) ELISA Kit 人抗α干扰素抗体 48T
Y166175 LKM(Human liver kidney microsome autoantibody) ELISA Kit 人抗肝肾微粒体抗体 48T
Y166176 ANA(Human Anti-nuclear Antibody) ELISA Kit 人抗核抗体 48T
Y166177 APF(Human anti-perinuclear factor) ELISA Kit 人抗核周因子抗体 48T
Y166178 CCP(Human anti-cyclic citrullinated peptide) ELISA Kit 人抗环胍氨酸肽抗体 48T
Y166179 AFA(Human anti-filaggrin antibody) ELISA Kit 人抗角蛋白丝聚集素/丝集蛋白抗体 48T
Y166180 CLA(Human Collagen autoantibody) ELISA Kit 人抗胶原蛋白抗体 48T
Y166181 Cry Beta(Human crystal proteins Beta) ELISA Kit 人晶体蛋白β 48T
Y166182 Cry Alpha(Human crystal proteins Alpha) ELISA Kit 人晶体蛋白α 48T
Y166183 MOG ELISA Kit (human) 抗髓鞘少树突胶质细胞糖蛋白(人) 96T
Y166184 AHA(Human anti-Histone antibody) ELISA Kit 人抗组蛋白抗体 48T
Y166185 Apl/APA(Human anti-phospholipid antibody) ELISA Kit 人抗磷脂抗体 48T
Y166186 IFA(Human intrinsic factor antibody) ELISA Kit 人抗内因子抗体 48T
Y166187 C1q(Human Anti-Complement 1q antibody) ELISA Kit 人抗补体1q抗体 48T
Y166188 EMAb(Human eye muscle antibody) ELISA Kit 人抗眼肌抗体 48T
Y166189 LSP(Human liver specific lipoprotein) ELISA Kit 人抗肝特异性脂蛋白抗体 48T
Y166190 LMA(Human anti-liver cell membrane antibody) ELISA Kit 人抗肝细胞膜抗体 48T
Y166191 ASMA(Human Anti-smooth muscle antibody) ELISA Kit 人抗平滑肌抗体 48T
Y166192 BB-Ab(Human anti-B burgdorleri antibody) ELISA Kit 人抗BB抗体 48T
Y166193 BP180-Ab(Human anti-Bullous Pemphigoid 180 antibody) ELISA Kit 人抗BP180抗体 48T
Y166194 anti-DNase B(Human anti-deoxyribonuclease B) ELISA Kit 人抗DNA酶B抗体 48T
Y166195 ANNA-1/Hu(Human neuronal nuclear autoantibody) ELISA Kit 人抗神经元核抗体1型/抗Hu抗体 48T
Y166196 Human anti-IgA antibody ELISA Kit 人抗IgA抗体 48T
Y166197 Human anti-Ku-antibody ELISA Kit 人抗Ku抗体 48T
Y166198 牛孕酮(PROG)ELISA KitHuman anti-Mi2-antibody ELISA Kit 人抗Mi2抗体 48T
Y166199 PL12/AlaRS(Human anti-PL12-antibody) ELISA Kit 人PL12抗体/抗丙氨酰tRNA合成酶 48T
Y166200 Human anti-PL7-antibody ELISA Kit 人PL7抗体/抗苏氨酰tRNA合成酶 48T
Y166201 PM-Scl/PM-1(Human polymyositis-sclerosis antibody) ELISA Kit 人抗多发性肌炎硬皮病抗体 48T
Y166202 anti-Q-Ab(Human anti-Q fever antibody) ELISA Kit 人抗Q热抗体 48T
Y166203 hnRNP/RA33(Human heterogeneous nuclear ribonucleoprotein compies/anti-RA33-antibody) ELISA Kit 人异质型核糖核蛋白复合物/抗RA33抗体 48T
Y166204 ANNA-2/Ri(Human neuronal nuclear autoantibody) ELISA Kit 人抗神经元核抗体2型/抗Ri抗体 48T
Y166205 Human anti-Sa-antibody ELISA Kit 人抗Sa抗体 48T
Y166206 PCA-1/Yo(Human purkinje cell autoantibody) ELISA Kit 人抗浦肯野细胞抗体/抗Yo抗体 48T
Y166207 AAA(Human anti-albumin antibody) ELISA Kit 人抗白蛋白抗体 48T
Y166208 ACA(Human anti-centrol and centrosome antibody) ELISA Kit 人抗中性粒/中心体抗体 48T
Y166209 EBMZ(Human epidermal basement membrane zone) ELISA Kit 人抗表皮细胞基底膜抗体 48T
Y166210 ATG(Human anti-thymocyte globulin) ELISA Kit 人抗胸腺细胞球蛋白 48T
Y166211 ABM-Ab(Human alveoli basement membrane zone antibody) ELISA Kit 人抗肺泡基底膜抗体 48T
Y166212 LC1(Human anti-liver cytosolantibody type 1) ELISA Kit 人抗肝细胞胞质1型抗体 48T
Y166213 gp210(Human Anti-glucoprotein 210) ELISA Kit 人抗核膜糖蛋白210抗体 48T
Y166214 ANA(Human anti-nucleolus antibody) ELISA Kit 人抗核仁抗体 48T
Y166215 28S rRNP(Human 28S ribosome RNP antibody) ELISA Kit 人28S抗核糖体抗体 48T
Y166216 RBC(Human anti-red cell antibody) ELISA Kit 人抗红细胞抗体 48T
Y166217 TPO-Ab(Human anti-Thyroid-Peroxidase antibody) ELISA Kit 人抗甲状腺过氧化物酶抗体 48T
Y166218 Human anti-macrophage antibody ELISA Kit 人抗巨噬细胞抗体 48T
Y166219 ALA/LCA(Human anti-lymphocytotoxic antibody) ELISA Kit 人抗淋巴细胞毒抗体 48T
Y166220 TA(Human anti-teichoic acid antibody) ELISA Kit 人抗磷壁酸抗体 48T
Y166221 APSA(Human Anti-phosphatidyl serine antibody) ELISA Kit 人抗磷脂酰丝氨酸抗体 48T
Y166222 AGA(Human anti-gliadin antibody) ELISA Kit 人抗麦胶蛋白/麦醇溶蛋白抗体 48T
Y166223 ABAb(Human anti-brain tissue antibody) ELISA Kit 人抗脑组织抗体 48T
Y166224 AhCGAb(Human anti-chorionic gonadotropin-antibody) ELISA Kit 人抗染色体抗体 48T
Y166225 AhCGAb(Human anti-chorionic gonadotropin-antibody) ELISA Kit 人抗人绒毛膜促性腺激素抗体 48T
Y166226 Human anti-cartilage-antibody ELISA Kit 人抗软骨抗体 48T
Y166227 Human anti-parotid duct antibody ELISA Kit 人抗腮腺管抗体 48T
Y166228 AMA IgA(Human anti-myelin antibody IgA) ELISA Kit 人抗髓磷脂抗体IgA 48T
Y166229 MCV(Human anti-myelin antibody IgA) ELISA Kit 人抗突变型瓜氨酸波形蛋白抗体 48T
Y166230 AMA IgA(Human anti-myelin antibody IgA) ELISA Kit 人抗髓磷脂抗体IgA 48T