1)包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
Y166231 MCV(Human anti-mutated citrullinated vimentin antibody) ELISA Kit 人抗突变型瓜氨酸波形蛋白抗体 48T
Y166232 ARA(Human anti-gastric parietal cell antibody) ELISA Kit 人抗网硬蛋白抗体 48T
Y166233 AGPA/PCA(Human anti-gastric parietal cell antibody) ELISA Kit 人抗胃壁细胞抗体 48T
Y166234 AIRA(Human anti-insulin receptor antibody) ELISA Kit 人抗胰岛素受体抗体 48T
Y166235 Apo A1(Human anti-apolipoprotein A1 antibody) ELISA Kit 人抗载脂蛋白抗体A1 48T
Y166236 ANA(Human anti-neutrophil antibody) ELISA Kit 人抗中性粒细胞抗体 48T
Y166237 ANGA(Human anti-neutrophil granules antibody) ELISA Kit 人抗中性粒细胞颗粒抗体 48T
Y166238 MAG Ab(Human anti-myelin associated glycoprotein antibody) ELISA Kit 人抗髓鞘相关糖蛋白抗体 48T
Y166239 GM1(Human anti-ganglioside antibody) ELISA Kit 人抗神经节苷脂抗体 48T
Y166240 SLE(Human systemic lupus erythematosus) ELISA Kit 人系统性红斑狼疮 48T
Y166241 AFA/snoRNP/U3RNP(Human anti-fibrillarin antibody) ELISA Kit 人抗核仁纤维蛋白抗体 48T
Y166242 OVA sIgE(Human ovalbumin specific IgE) ELISA Kit 人卵清蛋白特异性IgE 48T
Y166243 ACAST-D IV (Human autoantibodies against the C-terminal domain IV ) ELISA Kit 人抗钙蛋白酶抑素抗体 48T
Y166244 cmDNA(Human anti-cell membrane DNA antibody) ELISA Kit 人抗细胞膜DNA抗体 48T
Y166245 BA(Human Blocking antibody) ELISA Kit 人封闭抗体 48T
Y166246 SRP(Human signal recognization particle antibody) ELISA Kit 人抗信号识别颗粒抗体 48T
Y166247 GS-ANA(Human granulocyte specific antinuclear antibody) ELISA Kit 人粒细胞特异性抗核抗体 48T
Y166248 SCA(Human steroid producing cell autoantibody) ELISA Kit 人抗类固醇生成细胞抗体 48T
Y166249 THAA(Human thyroid hormone autoantibodies) ELISA Kit 人甲状腺非肽激素抗体 48T
Y166250 CAM-ab(Human anti-calmodulin specific antibody) ELISA Kit 人抗钙调素特异抗体 48T
Y166251 OVA sIgG(Human ovalbumin specific IgG) ELISA Kit 人卵清蛋白特异性IgG 48T
Y166252 ANG- I R(Human angiotension I receptor Antibody) ELISA Kit 人血管紧张素Ⅰ受体抗体 48T
Y166253 AT II R1(Human angiotension II receptor 1 Antibody) ELISA Kit 人血管紧张素Ⅱ受体1抗体 48T
Y166254 AT2R-Ab(Human Angiotensin II Receptor 2 antibody) ELISA Kit 人血管紧张素Ⅱ受体2抗体 48T
Y166255 PsmAb(Human anti-presynaptic membrane antibody) ELISA Kit 人抗突触前膜抗体 48T
Y166256 TTN(Human Anti-titin Antibody) ELISA Kit 人抗肌联蛋白抗体 48T
Y166257 GM-CSF Ab(Human anti-Granulocyte-Macrophage Colony Stimulating Factor antibody) ELISA Kit 人粒细胞巨噬细胞集落刺激因子抗体 48T
Y166258 Surv(Human anti-Survivin antibody) ELISA Kit 人抗存活素抗体/生存蛋白 48T
Y166259 tTG-IgA(Human Anti-tissue transglutaminase IgA) ELISA kit 人抗组织转谷氨酰胺酶抗体IgA 48T
Y166260 Nogo-A Ab(Human anti-Nogo-A antibody) ELISA Kit 人NOGO-A抗体 48T
Y166261 FN(Human Fibronectin) ELISA Kit 人纤连蛋白 48T
Y166262 LN(Human Laminin) ELISA KIT 人层连蛋白/板层素 48T
Y166263 Col III (Human Collagen Type III ) ELISA KIT 人Ⅲ型胶原 48T
Y166264 Col IV (Human Collagen Type IV ) ELISA KIT 人Ⅳ型胶原 48T
Y166265 HA(Human Hyaluronic acid) ELISA Kit 人透明质酸 96T
Y166266 P I CP(Human procollagen III N-terminal peptide) ELISA Kit 人Ⅰ型前胶原羧基端肽 48T
Y166267 Col I (Human Collagen Type I ) ELISA Kit 人Ⅰ型胶原 48T
Y166268 Col II (Human Collagen Type II ) ELISA Kit 人Ⅱ型胶原 48T
Y166269 P III NP(Human N-terminal procollagen III propeptide) ELISA Kit 人Ⅲ型前胶原肽 48T
Y166270 CCK(Human cholecystokinin) ELISA Kit 人胆囊收缩素/肠促胰酶肽 48T
Y166271 MTL(Human Motilin) ELISA Kit 人血管活性肠肽 48T
Y166272 Human Amylin ELISA Kit 人胰淀素 48T
Y166273 MDA(Human malondialchehyche) ELISA Kit 人丙二醛 48T
Y166274 eNOS-3(Human Alpha1-Acid glycoprotein) ELISA Kit 人内皮型一氧化氮合成酶3 48T
Y166275 Alpha1-AGP(Human Alpha1-Acid glycoprotein) ELISA Kit 人α1酸性糖蛋白 48T
Y166276 TAP(Human trypsinogen activation peptide) ELISA Kit 人胰蛋白酶原激活肽 48T
Y166277 CCK-8(Human cholecystokinin octapeptide) ELISA Kit 人胆囊收缩素/缩胆囊素八肽 96T
Y166278 GsaR(Human gastrin receptor) ELISA Kit 人促胃液素受体 48T
Y166279 Human Peptide YY ELISA Kit 人多肽YY 48T
Y166280 Human Secretin ELISA Kit 人促胰液素/胰泌素 48T
Y166281 HCB II (Human Collagen-like Bioprotein II ) ELISA Kit 人胶原蛋白Ⅱ 48T
Y166282 ProGRP(Human pro-gastrin-releasing peptide) ELISA Kit 人胃泌素释放肽前体 48T
Y166283 GRP(Human gastrin-reliasing peptide) ELISA Kit 人胃泌素释放多肽 48T
Y166284 GIP(Human gastric inhibitory polypeptide) ELISA Kit 人胃抑素 48T
Y166285 CagA(Human Cytotoxin-associated protein) ELISA Kit 人细胞毒素相关蛋白A 48T
Y166286 Hp-IgM(Human Helicobacter pylori IgM) ELISA Kit 人幽门螺旋杆菌IgM 48T
Y166287 NTX(Human cross linked N-telopeptide of type I collagen) ELISA Kit 人Ⅰ型胶原N末端肽 48T
Y166288 HABP(Human Hya]uronate binding protein) ELISA Kit 人透明质酸结合蛋白 48T
Y166289 GM(Human Gastric Mucin) ELISA Kit 人胃粘液素 48T
Y166290 GGT(Human gamma glutamyl transpeptidase) ELISA Kit 人γ谷氨酰转移酶 48T
Y166291 Hp-IgG(Human Helicobacter pylori IgG ) ELISA Kit 人幽门螺旋菌IgG 48T
Y166292 CA(Human Cholic acid) ELISA Kit 人胆酸 48T
Y166293 HHC II (uman heparin cofactor II ) ELISA Kit 人肝素辅因子Ⅱ 48T
Y166294 GDH/GLDH(Human Glutamate dehydrogenase) ELISA Kit 人谷氨酸脱氢酶 48T
Y166295 GCA(Human gastrin cell antibody) ELISA Kit 人胃泌素细胞抗体 48T
Y166296 FRA(Human fibronection related antigen) ELISA Kit 人纤维连接素相关抗原 48T
Y166297 HELIX- II (Human type II collagen helical peptide) ELISA Kit 人Ⅱ型胶原螺旋肽 48T
Y166298 HPA(Human Heparanase) ELISA kit 人乙酰肝素酶 48T
Y166299 HL(Human hepatic lipase) ELISA Kit 人肝脂酶 48T
Y166300 GOT/GPT(Human Aspartate aminotransferase) ELISA Kit 人天门冬氨酸转氨酶/谷草转氨酶 48T
Y166301 ALT/GPT(Human Alanine Aminotransferase) ELISA Kit 人丙氨酸转氨酶/谷丙转氨酶 48T
Y166302 MUC5AC(Human Mucin-5 subtype AC) ELISA Kit 人粘蛋白/粘液素5AC 48T
Y166303 CTX- I (Human C-telopeptide of type I collagen) ELISA Kit 人Ⅰ型胶原C端肽 48T
Y166304 I CTP(Human Cross-linked Carboxy-terminal telopeptide of type I collagen) ELISA Kit 人Ⅰ型胶原交联羧基末端肽 48T
Y166305 P I NP(Human procollagen I N-terminal peptide) ELISA Kit 人Ⅰ型前胶原N端前肽 48T
Y166306 TGM2(human transglutaminase 2C polypeptide) ELISA Kit 人转谷氨酰胺酶2C多肽 48T
Y166307 MUC7(Human Mucin-7) ELISA Kit 人粘蛋白/粘液素7 48T
Y166308 HP-CagA-IgG(Human Helicobacter pylori cytotoxin-associated gene A protein IgG) ELISA Kit 人幽门螺杆菌细胞毒素相关基因蛋白A-IgG 48T
Y166309 SP-A(Human Pulmonary surfatcant-associated protein A) ELISA Kit 人肺表面活性物质相关蛋白A 48T
Y166310 AACT(Human Alpha1 Antichymotrypsin) ELISA Kit 人α1抗胰糜蛋白酶 48T
Y166311 LRP(Human Lung resistance-related protein) ELISA Kit 人肺耐药蛋白 48T
Y166312 Cpn-Ab(Human Chlamydia pneumoniae antibody) ELISA Kit 人肺炎衣原体抗体 48T
Y166313 MP-Ab(Human Mycoplasma pneumoniae antibody) ELISA Kit 人肺炎支原体抗体 48T
Y166314 SP-A(Human Surfactant Protein A) ELISA Kit 人表面活性蛋白A 48T
Y166315 SP-B(Human Pulmonary surfatcant-associated protein B) ELISA Kit 人肺表面活性物质相关蛋白B 48T
Y166316 SP-C(Human Pulmonary surfatcant-associated protein C) ELISA Kit 人肺表面活性物质相关蛋白C 48T
Y166317 SP-D(Human Pulmonary surfatcant-associated protein D) ELISA Kit 人肺表面活性物质相关蛋白D 48T
Y166318 OSM(Human Oncostatin-M) ELISA KIT 人抑瘤素M 48T
Y166319 TIMP-2(Human tissue inhibitors of metalloproteinase 2) ELISA Kit 人基质金属蛋白酶抑制因子2 48T
Y166320 TIMP-3(Human tissue inhibitors of metalloproteinase 3) ELISA Kit 人基质金属蛋白酶抑制因子3 48T
Y166321 TIMP-4(Human tissue inhibitors of metalloproteinase 4) ELISA Kit 人基质金属蛋白酶抑制因子4 48T
Y166322 CEA/CD66(Human carcinoembryonic antign) ELISA Kit 人癌胚抗原 48T
Y166323 AFP(Human Alpha-fetoprotein) ELISA Kit 人甲种胎儿球蛋白/甲胎蛋白 48T
Y166324 Human ovarian cancer marker-CA125 牛组胺(HIS)ELISA KitELISA Kit 人卵巢癌标志物CA125 48T
Y166325 Human mammary carcinoma Marker-CA153 ELISA Kit 人乳腺癌标志物-CA153 48T
Y166326 Human Gastrointestinalcancer Marker-CA199 ELISA Kit 人胃肠癌标志物CA199 48T