1)包被:用0.05M PH9.牰碳酸盐包被缓冲液将抗体稀释至蛋白质含量为1~10μg/ml。在每个聚苯乙烯板的反应孔中加0.1ml,4℃过夜。次日,弃去孔内溶液,用洗涤缓冲液洗3次,每次3分钟。(简称洗涤,下同)。
结果判定:可于白色背景上,直接用肉眼观察结果:反应孔内颜色越深,阳性程度越强,阴性反应为无色或极浅,依据所呈颜色的深浅,以“+”、“-”号表示。也可测O·D值:在ELISA检测仪上,于450nm(若以ABTS显色,则410nm)处,以空白对照孔调零后测各孔O·D值,若大于规定的阴性对照OD值的2.1倍,即为阳性。
用包被缓冲液将已知抗原稀释至1~10μg/ml,每孔加0.1ml,4℃过夜。次日洗涤3次。加一定稀释的待检样品(未知抗体)0.1ml于上述已包被之反应孔中,置37℃孵育1小时,洗涤。(同时做空白、阴性及阳性孔对照)于反应孔中,加入新鲜稀释的酶标第二抗体(抗抗体)0.1ml,37℃孵育30-60分钟,洗涤,最后一遍用DDW洗涤。其余步骤同“双抗体夹心法”的4、5、6。
Y166437 Dyn(Human dynorphin) ELISA Kit 人强啡肽 48T
Y166438 NKB(Human Neurokinins B) ELISA Kit 人神经激肽B 48T
Y166439 NT(Human Neurotensin) ELISA Kit 人神经降压素 48T
Y166440 KP(Human kaliuretic peptide) ELISA Kit 人利钾尿肽 48T
Y166441 NF(Human neurofilament protein) ELISA Kit 人神经丝蛋白 48T
Y166442 MAP-2(Human microtubule-associated protein 2) ELISA Kit 人微管相关蛋白2 48T
Y166443 PACAP(Human pituitary adenylate cyclase activating polypeptide) ELISA Kit 人垂体腺苷酸环化酶激活肽 48T
Y166444 AVP(Human arginine vasopressin) ELISA Kit 人精氨酸加压素 48T
Y166445 p2(Human myelin protein 2) ELISA Kit 人神经髓鞘蛋白 48T
Y166446 ANF(Human atrial natriuretic factor) ELISA Kit 人心纳素 48T
Y166447 6-OHDA(Human 6-hydroxydopamine) ELISA Kit 人6-羟多巴胺 48T
Y166448 DSIP(Human delta sleep-inducing peptide) ELISA Kit 人促睡眠肽 48T
Y166449 OX-B(Human Orexin B) ELISA Kit 人食欲素/阿立新B 48T
Y166450 NAIP(Human neuronal apoptosis inhibitory protein) ELISA Kit 人神经元凋亡抑制蛋白 48T
Y166451 INH(Human Inhibin) ELISA Kit 人抑制素 48T
Y166452 Alpha-EP(Human Alpha-Endomorphin) ELISA Kit 人α-内吗啡肽 48T
Y166453 EM-2(Human endomorphin-2) ELISA Kit 人内吗啡肽-2 48T
Y166454 D2R(Human dopamine D2 receptor) ELISA Kit 人多巴胺D2受体 48T
Y166455 ANP(Human Atrial Natriuretic Peptide) ELISA Kit 人心钠肽 48T
Y166456 NRG-1(human Neuregulin 1) ELISA Kit 人神经调节蛋白1 48T
Y166457 ACV-A(Human Activin A) ELISA Kit 人活化素A 48T
Y166458 ALCAM(Human Activated Leukocyte Cell Adhesion Molecule) ELISA Kit 人白细胞活化黏附因子 48T
Y166459 BDNF(Human Brain derived neurotrophic facor) ELISA Kit 人脑源性神经营养因子 48T
Y166460 E-Cad(Human E-Cadherin) ELISA Kit 人E钙粘着蛋白/上皮性钙黏附蛋白 48T
Y166461 SLC/CCL21(Human secondary lymphoid-tissue chemokine) ELISA Kit 人二级淋巴组织趋化因子 48T
Y166462 XCR1(Human XC-chemokine receptor 1) ELISA Kit 人XC趋化因子受体1 48T
Y166463 CXCR1(Human CXC-chemokine receptor 1) ELISA Kit 人CXC趋化因子受体1 48T
Y166464 CD30(Human Cluster of differentiation 30) ELISA Kit 人CD30分子 48T
Y166465 CNTF(Human Ciliary Neurotrophic Factor) ELISA Kit 人睫状神经营养因子 48T
Y166466 EG-VEGF(Human Endocrine gland vascular endothelial growth factor) ELISA Kit 人内分泌腺来源的血管内皮生长因子 48T
Y166467 ECF(Human eosinophil chemotactic factor) ELISA Kit 人鼠嗜酸粒细胞趋化因子 48T
Y166468 CCL1(Human Eotaxin 1) ELISA Kit 人嗜酸粒细胞趋化蛋白 48T
Y166469 CD62E(Human E-Selectin) ELISA kit 人E选择素 48T
Y166470 FAS/CD95(Human Factor-related Apoptosis) ELISA Kit 人凋亡相关因子 48T
Y166471 FASL(Human Factor-related Apoptosis ligand) ELISA Kit 人凋亡相关因子配体 48T
Y166472 aFGF-1(Human acidic fibroblast growth factor 1) ELISA Kit 人酸性成纤维细胞生长因子1 48T
Y166473 bFGF-4(Human basic fibroblast growth factor 4) ELISA Kit 人碱性成纤维细胞生长因子4 48T
Y166474 bFGF-6(Human basic fibroblast growth factor 6) ELISA Kit 人碱性成纤维细胞生长因子6 48T
Y166475 bFGF-9(Human basic fibroblast growth factor 9) ELISA Kit 人碱性成纤维细胞生长因子9 48T
Y166476 Human fractalkine/CX3CL1 ELISA Kit 人趋化因子 48T
Y166477 NAP-2/CXCL7(Human neutrophil activating protein-2) ELISA Kit 人中性粒细胞趋化蛋白2 48T
Y166478 G-CSF(Human Granulocyte Colony Stimulating Factor) ELISA Kit 人粒细胞集落刺激因子 48T
Y166479 GDNF(Human glial cell line-derived neurotrophic factor) ELISA KIT 人胶质细胞系来源的神经营养因子 48T
Y166480 GM-CSF(Human Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit 人粒细胞巨噬细胞集落刺激因子 48T
Y166481 HGF(Human hepatocyte growth factor) ELISA kit 人肝细胞生长因子 48T
Y166482 ICAM-1/CD54(Human intercellular adhesion molecule 1) ELISA Kit 人细胞间粘附分子1 48T
Y166483 IFN- Gamma(Human Interferon Gamma) ELISA Kit 人γ干扰素 48T
Y166484 IGF-1(Human insulin-like growth factors 1) ELISA Kit 人胰岛素样生长因子1 48T
Y166485 IGF-2(Human insulin-like growth factors 2) ELISA Kit 人胰岛素样生长因子2 48T
Y166486 IGFBP-1(Human insulin-like growth factors binding protein 1) ELISA Kit 人胰岛素样生长因子结合蛋白1 48T
Y166487 IGFBP2(Human insulin-like growth factors binding protein 2) ELISA Kit 人胰岛素样生长因子结合蛋白2 48T
Y166488 IGFBP-3(Human insulin-like growth factors binding protein 3) ELISA Kit 人胰岛素样生长因子结合蛋白3 48T
Y166489 IGFBP-4(Human insulin-like growth factors binding protein 4) ELISA Kit 人胰岛素样生长因子结合蛋白4 48T
Y166490 IL-10(Human Interleukin 10) ELISA KIT 人白介素10 48T
Y166491 IL-11(Human Interleukin 11) ELISA KIT 人白介素11 48T
Y166492 IL-12/P70(Human Interleukin 12) ELISA KIT 人白介素12 48T
Y166493 IL-13(Human Interleukin 13) ELISA KIT 人白介素13 48T
Y166494 IL-16(Human Interleukin 16) ELISA KIT 人白介素16 48T
Y166495 IL-1sR I (Human soluble interleukin-1 receptor ) ELISA kit 人白介素1可溶性受体Ⅰ 48T
Y166496 IL-1sR II (Human soluble interleukin-1 receptor II ) ELISA kit 人白介素1可溶性受体Ⅱ 48T
Y166497 IL-1 Alpha (Human Interleukin 1 Alpha) ELISA Kit 人白介素1α 48T
Y166498 IL-2(Human Interleukin 2) ELISA kit 人白介素2 48T
Y166499 IL-2sR Alpha/CD25(Human soluble interleukin-2 receptor) ELISA kit 人白介素2可溶性受体α链 48T
Y166500 IL-2sR Beta (Human Interleukin 2) ELISA kit 人白介素2可溶性受体β链 48T
Y166501 IL-3(Human Interleukin 3) ELISA KIT 人白介素3 48T
Y166502 IL-4(Human Interleukin 4) ELISA KIT 人白介素4 48T
Y166503 IL-5(Human Interleukin 5) ELISA KIT 人白介素-5 48T
Y166504 IL-6(Human Interleukin 6) ELISA KIT 人白介素6 48T
Y166505 IL-8/CXCL8(Human Interleukin 8) ELISA KIT 人白介素8 48T
Y166506 IL-9(Human Interleukin 9) ELISA KIT 人白介素9 48T
Y166507 Human L-Selectin ELISA kit 人L选择素 48T
Y166508 MCP-1/CCL2/MCAF(Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor) ELISA kit 人单核细胞趋化蛋白1 48T
Y166509 MCP-2/CCL8(Human monocyte chemotactic protein 2) ELISA kit 人单核细胞趋化蛋白2 48T
Y166510 MCP-3/CCL7(Human monocyte chemotactic protein 3) ELISA kit 人单核细胞趋化蛋白3 48T
Y166511 MIC-1 human ELISA kit 人巨噬细胞抑制因子-1 ELISA 试剂盒 96T
Y166512 M-CSF(Human Macrophage Colony-Stimulating Factor) ELISA kit 人巨噬细胞集落刺激因子 48T
Y166513 MDC/CCL22(Human Macrophage-Derived Chemokine) ELISA kit 人巨噬细胞来源的趋化因子 48T
Y166514 MIP-1 Alpha/CCL3(Human Macrophage Inflammatory Protein-1 Alpha) ELISA kit 人巨噬细胞炎性蛋白1α 48T
Y166515 MIP-1 Beta/CCL4(Human Macrophage Inflammatory Protein-1 Beta) ELISA kit 人巨噬细胞炎性蛋白1β 48T
Y166516 MIP-3 Alpha/CCL20(Human Macrophage Inflammatory Protein-3 Alpha) ELISA kit 人巨噬细胞炎性蛋白3α 48T
Y166517 MIP-3 Beta/ELC/CCL19(Human Macrophage Inflammatory Protein-3 Beta) ELISA kit 人巨噬细胞炎性蛋白3β 48T
Y166518 NGF- Beta(Human Nerve growth factor- Beta) ELISA kit 人的神经生长因子-β 96T
Y166519 NGF(Human Nerve growth factor) ELISA kit 人的神经生长因子 48T
Y166520 NT-3 ELISA Kit 人神经营养因子3 48T
Y166521 HNT-4(uman Neurotrophin 4) ELISA KIT 人神经营养因子4 48T
Y166522 PDGFsR- Alpha(Human Platelet-Derived Growth Factor Soluble Receptor Alpha) ELISA kit 人血血小板衍生生长因子可溶性受体α 48T
Y166523 PDGF-AB(Human Platelet-Derived Growth Factor AB) ELISA kit 人血血小板衍生生长因子AB 48T
Y166524 P-Selectin(Human P-Selectin/CD62P/GMP140) ELISA kit 人P选择素 48T
Y166525 RANTES/CCL5(Human regulated on activation in normal T-cell expressed and secreted) ELISA kit 人正常T细胞表达和分泌因子 48T
Y166526 sCD30L(Human Soluble Cluster of differentiation 30 ligand) ELISA Kit 人可溶性CD30配体 48T
Y166527 sCD40L(Human Soluble Cluster of differentiation 40 ligand) ELISA Kit 人可溶性CD40配体 48T
Y166528 SCF/MGF(Human Stem cell factor/mast cell growth factor) ELISA kit 人干细胞因子/肥大细胞生长因子 48T
Y166529 SCFR(Human Stem Cell Factor Receptor) ELISA kit 人干细胞因子受体 48T
Y166530 SDF-1 Beta/CXCL12(Human Stromal cell derived factor 1 Beta) ELISA kit 人基质细胞衍生因子1β 48T
Y166531 TGF- Alpha(Human transforming growth factor Alpha) ELISA Kit 人转化生长因子α 48T
Y166532 TGF- Beta1(Human Transforming Growth factor Beta1) ELISA kit 人转化生长因子β1 48T
Y166533 TNFsR- I (Human Tumor necrosis factor soluble receptor I ) ELISA KIT 人肿瘤坏死因子可溶性受体Ⅰ 48T
Y166534 TNFsR- II (Human Tumor necrosis factor soluble receptor II ) ELISA KIT 人肿瘤坏死因子可溶性受体Ⅱ 48T
Y166535 TNF- Alpha(Human Tumor necrosis factor Alpha) ELISA KIT 人肿瘤坏死因子α 48T
Y166536 TNF- Beta(Human Tumor necrosis factor Beta) ELISA KIT 人肿瘤坏死因子β 48T
Y166537 TRAIL-R1(Human tumor necrosis factor-related apoptosis-inducing ligand 1) ELISA Kit 人肿瘤坏死因子相关凋亡诱导配体1 48T
Y166538 TRAIL-R3(Human tumor necrosis factor-related apoptosis-inducing ligand 3) ELISA Kit 人肿瘤坏死因子相关凋亡诱导配体3 48T
Y166539 TRAIL-R4(Human tumor necrosis factor-related apoptosis-inducing ligand 4) ELISA Kit 人肿瘤坏死因子相关凋亡诱导配体4 48T
Y166540 sTRAIL(Human soluble tumor necrosis factor-related apoptosis inducing ligand) ELISA KIT 人可溶性肿瘤坏死因子相关凋亡诱导配体 48T
Y166541 VCAM-1/CD106(Human Vascuolar cell adhesion molecule 1) ELISA kit 人血管内皮细胞粘附分子1 48T
Y166542 VEGF(Human Vascular Endothelial cell Growth Factor) 犬白介素1β(IL-1β)ELISA KitELISA KIT 人血管内皮细胞生长因子 48T
Y166543 VEGF-B(Human Vascular Endothelial cell Growth Factor B) ELISA KIT 人血管内皮细胞生长因子B 48T
Y166544 VEGF-C(Human Vascular Endothelial cell Growth Factor C) ELISA KIT 人血管内皮细胞生长因子C 48T