抗体的溶解方法

抗体的溶解方法
方法1:国内抗体(冻干粉)已经包含了0.01M PBS、1% BSA和0.1% 防腐剂(叠氮钠或庆大霉素),您只需要加入灭菌的双蒸水就行了。抗体溶解后,置于-20℃保存,最好分装后使用,避免反复冻融,可保证至少1年有效;
方法2:也可以只加入一半体积的双蒸水,再用一半体积的甘油补足,置于-20℃保存,这样抗体不会冻,有利于抗体的稳定。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:
(1) Reconstituting by directly adding 100uL of sterile, distilled water.
(2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

 

阅读67次
关注
最新动态

相关产品

当前位置: 森贝伽生物 动态 抗体的溶解方法

关注

拨打电话

留言咨询