方案摘要
方案下载| 应用领域 | |
| 检测样本 | |
| 检测项目 | |
| 参考标准 |
Porcine(LDH)ELISA Kit Porcine Lactate Dehydrogenase (LDH) ELISA Kit FOR RESEARCH USE ONLY Purpose This kit allows for the determination of LDH concentrations in Porcineserum, blood plasma, and other biological fluids. Principle of the assay The kit assay Porcine LDH level in the sample，use Purified Porcine LDHantibody to coat microtiter plate wells, make solid-phase antibody, then addLDH to wells, Combined LDH antibody which With HRP labeled, becomeantibody - antigen - enzyme-antibody complex, after washing Completely, AddTMB substrate solution,TMB substrate becomes blue color At HRPenzyme-catalyzed, reaction is terminated by the addition of a sulphuric acidsolution and the color change is measured spectrophotometrically at awavelength of 450 nm. The concentration of LDH in the samples is thendetermined by comparing the O.D. of the samples to the standard curve.
文献贡献者
小鼠前列腺素E2(PGE2)ELISA试剂盒操作步骤
黑色素染色液(硫酸亚铁法)染色步骤及注意事项
小鼠糖缺失性转铁蛋白(CDT)ELISA试剂盒实验方案
相关产品
关注
拨打电话
留言咨询
