The isoelectric point (IEP) is the pH value where the zeta potential is zero.can be determined by the Nicomp 380 ZLS by titrating the sample and recording zeta potential as a function of pH.
等电点可以通过滴定样品PH值，再用Nicomp 380 ZLS 记录Zeta电位，Zeta电位为0时的PH值就是等电点。
Coffee-mate powder was prepared by mixing 0.1 g in 200 mL of DI water. The sample was placed on a magnetic stirring plate and mixed constantly during the study, with a newly calibrated pH probe immersed in the suspension.
将奶精粉与去离子水以0.1g：100 mL混合。样品置于磁力搅拌盘内一直搅拌，并插入一个全新的PH 探针。
The pH was altered by adding drops of 0.1N hydrochloric acid. After the pH stabilized a sample was removed by a syringe and injected into the zeta potential cell. Three zeta potential measurements were performed and the average value were plotted vs pH. The results are shown in the graph below.
随着0.1N 的盐酸加入，PH会发生改变。当PH稳定后用注射器移取样品到Zeta电位测量池。测量三次取其平圴值，与相对应的PH值作图，如下所示：
The IEP of the Coffee-mate suspension in this study was determined to be at pH 4.26.
此次研究中奶精粉悬浮液的等电点为PH 4.26
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