Ras蛋白脑组织同源类似物RHEB蛋白抗体

本公司是最专业Ras蛋白脑组织同源类似物RHEB蛋白抗体供应商，提供Ras蛋白脑组织同源类似物RHEB蛋白抗体的报价，泛素样蛋白Sumo2/3抗体咨询，

英文名称  Anti-RHEB

中文名称  Ras蛋白脑组织同源类似物RHEB蛋白抗体

别    名  GTP-binding protein Rheb; MGC111559; Ras homolog enriched in brain 2; Ras homolog enriched in brain; RHEB 2; RHEB-2 RHEB2; RHEB_HUMAN.

浓    度  1mg/1ml

规 格  0.1ml/100μg  0.2ml/200μg

Ras蛋白脑组织同源类似物RHEB蛋白抗体概述：

B淋巴细胞在抗原的刺激下，能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的，不可能持续分化增殖下去，因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞，再进一步克隆化，这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力，又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chicken, Cow, Horse

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 神经生物学  

蛋白分子量  predicted molecular weight: 20kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RHEB

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Function : Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.

Subunit : Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2.

Subcellular Location : Cell membrane; Lipid-anchor; Cytoplasmic side.

Tissue Specificity : Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

Post-translational modifications : Farnesylation is important for efficiently activating mTORC1-mediated signaling.

Ras蛋白脑组织同源类似物RHEB蛋白抗体Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation (By similarity).

Similarity : Belongs to the small GTPase superfamily. Rheb family.

Database links : UniProtKB/Swiss-Prot: Q15382.1