磷酸化周期素B1抗体

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英文名称  Anti-Phospho-Cyclin B1 (Ser126)

中文名称  磷酸化周期素B1抗体

别    名  Cyclin B1 (phospho S126); Cyclin B1 (phospho-Ser126); p-Cyclin B1 (S126); p-Cyclin B1 (Ser126); p-Cyclin B1 (phospho S126); CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.

浓    度  1mg/1ml

规 格  0.1ml/100μg  

磷酸化周期素B1抗体概述：

B淋巴细胞在抗原的刺激下，能够分化、增殖形成具有针对这种抗原分泌特异性抗体的能力。B细胞的这种能力和量是有限的，不可能持续分化增殖下去，因此产生免疫球蛋白的能力也是极其微小的。将这种B细胞与非分泌型的骨髓瘤细胞融合形成杂交瘤细胞，再进一步克隆化，这种克隆化的杂交瘤细胞是既具有瘤细胞的无限分裂的能力，又具有产生特异性抗体的B淋巴细胞的能力。将这种克隆化的杂交瘤细胞进行培养或注入小鼠腹水内即可获得大量的高效、单一的特异性抗体。这种技术即称为单克隆抗体技术。

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human  

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 细胞周期蛋白  

蛋白分子量  predicted molecular weight: 48kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser126

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Function : Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Subunit : Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization.

Subcellular Location : Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.

Post-translational modifications : Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.

Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

磷酸化周期素B1抗体Similarity : Belongs to the cyclin family. Cyclin AB subfamily.

Database links : UniProtKB/Swiss-Prot: P14635.1

主要出现在G2期。Cyclin B是细胞周期调节必不可少的条件。 细胞周期素B1是细胞周期调节因子，它的异常表达将导致细胞周期发生紊乱，致使肿瘤形成.