磷酸化波形蛋白抗体

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英文名称  Anti-Phospho-Vimentin (Ser56)

中文名称  磷酸化波形蛋白抗体

别    名  FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 发育生物学 神经生物学 信号转导 干细胞 细胞骨架 肿瘤细胞生物标志物  

蛋白分子量  predicted molecular weight: 51kDa

性    状  Lyophilized or Liquid

磷酸化波形蛋白抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser56

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Function : Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Subunit : Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain).Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B.

Subcellular Location : Cytoplasm.

Tissue Specificity : Highly expressed in fibroblasts, somev expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Post-translational modifications : Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.

磷酸化波形蛋白抗体Similarity : Belongs to the intermediate filament family.

Database links : UniProtKB/Swiss-Prot: P08670.4

Vimentin—波形蛋白。是五种主要的中间丝之一，存在于各种正常和病理性间质来源的组织，如纤维母细胞、内皮细胞、淋巴细胞等正常细胞和肉瘤、淋巴瘤、黑色素瘤等肿瘤。波形蛋白是负责维持细胞骨架完整性的蛋白之一。