磷酸化桩蛋白Paxillin抗体

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英文名称  Anti-Phospho-Paxillin(Tyr31)

中文名称  磷酸化桩蛋白Paxillin抗体

别    名  PXN(phospho Y31); Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; PAXI_HUMAN.

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chicken, Cow, Horse, Sheep  

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 细胞凋亡 转录调节因子 激酶和磷酸酶 细胞表面分子  

蛋白分子量  predicted molecular weight: 64kDa

性    状  Lyophilized or Liquid

磷酸化桩蛋白Paxillin抗体随着国内抗体行业的崛起，我国自主品牌的抗体，都蛮多了，质量也不错，技术在逐渐成熟，价格也比国外的廉价的多，受到国内顾客的热捧，我们公司为了方便客户，特推出阴离子转运蛋白-1抗体规格15ug、30ug、50ug、100ug，15ug和30ug等，我们全国包邮，大中城市免费快递。抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Function : Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Subunit : Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB.

Subcellular Location : Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex (By similarity). Note=Colocalizes with integrins at the cell periphery (By similarity).

Post-translational modifications : Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion.

Similarity : Belongs to the paxillin family.

Contains 4 LIM zinc-binding domains.

Database links : UniProtKB/Swiss-Prot: P49023.3

磷酸化桩蛋白Paxillin抗体Paxillin是一种分子量为68KDa的局部粘附蛋白，参与肿瘤细胞的粘附与转移。主要用于各种恶性肿瘤的研究。 Paxillin 蛋白是胞外基质细胞粘附（粘着斑）位点上肌动蛋白-膜附着的细胞骨架蛋白。 Paxillin 在整合蛋白的信号传导中起着非常重要的作用，整合蛋白介导的细胞骨架的再组织需要，paxillin 酪氨酸残基的磷酸化。 Paxillin 被粘着斑激酶 (FAK)在其118位的酪氨酸残基处磷酸化。已经证明，paxillin 118位和31位两个酪氨酸的磷酸化对于MM1癌细胞的迁移是重要的。