cGMP依赖性蛋白激酶1抗体

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英文名称  Anti-Prkg1/cGKI

中文名称  cGMP依赖性蛋白激酶1抗体

别    名  cGKI alpha; CGKI; cGKI beta; cGMP-dependent protein kinase 1; KGPB; pkg; PRKG1; PRKG1B; PRKGR1A; PRKGR1B.

浓    度  1mg/1ml

规 格  0.1ml/100μg  0.2ml/200μg

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

产品类型  一抗    

研究领域  心血管 信号转导 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 74kDa

性    状  Lyophilized or Liquid

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免 疫 原  KLH conjugated synthetic peptide derived from human Prkg1/cGKI corresponding to amino acids 582-628.

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation.

Subunit : Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A.

Subcellular Location : Cytoplasm.

Tissue Specificity : Primarily expressed in lung and placenta.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

cGMP依赖性蛋白激酶1抗体Contains 1 AGC-kinase C-terminal domain.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 protein kinase domain.