供货周期: | 现货 |
品牌: | GenWay |
规格: | 0.1ml/100μg和0.2ml/200μg等规格 |
货号: | |
CAS号: |
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英文名称 Anti-capsid protein
中文名称 猪圆环Ⅱ型衣壳蛋白
别 名 ORF1; ORF2 (PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.)
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 PCV2
产品类型 一抗
研究领域 细胞生物 免疫学 细菌及
蛋白分子量 predicted molecular weight: 26kDa
性 状 Lyophilized or Liquid
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免 疫 原 KLH conjugated synthetic peptide derived from PCV2 capsid protein
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required.
Function : Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope.
Subunit : Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus.
Subcellular Location : Host nucleus. Virion (Potential).
Similarity : Belongs to the circoviridae capsid protein family.
Database links : UniProtKB/Swiss-Prot: O56129.1
猪圆环Ⅱ型衣壳蛋白猪圆环-PCV-2是一种嗜单核-巨噬细胞性的,属于圆环科圆环属的成员,根据抗原性及基因组成,分为PCVl和PCV2两种,其中PCV2对猪具有致病性,PCV2(囊膜衣壳蛋白(Cap))主要侵害免疫系统,导致感染猪免疫抑制,降低机体对其他病原体的抵抗力和疫苗的反应性,容易导致其他和细菌的继发感染,使死亡率明显升高。
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