供货周期: | 现货 |
品牌: | ATCC |
型号: | 1000000个细胞数/瓶 |
货号: | Cell-3483 |
CAS号: | 说明书有详尽的标注 |
人黑色素瘤高转移细胞亚系;A375sci
成纤维细胞样
贴壁生长
该细胞属专利保藏,其特征特性尚未公开。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1:3传代,3-4天传1次
C3
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)人黑色素瘤高转移细胞亚系;A375sci冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到人黑色素瘤高转移细胞亚系;A375sci后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
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