供货周期: | 现货 |
品牌: | ATCC |
型号: | 1000000个细胞数/瓶 |
货号: | Cell-3793 |
CAS号: | 说明书有详尽的标注 |
大鼠腹水癌细胞;Walker/LLC-WRC 256
淋巴母细胞样
悬浮生长
Walker 256是一株大鼠腹水癌细胞系。该细胞注入大鼠腹腔能快速形成腹水瘤,是研究抗肿瘤药物体内模型的极好材料。
RPMI 1640 (w/o Hepes) 10%FBS
1:3传代,2-3天传一代
C180
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)大鼠腹水癌细胞;Walker/LLC-WRC 256每冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到大鼠腹水癌细胞;Walker/LLC-WRC 256后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
A14450 Metaxin 3蛋白(MTX3)酶联免疫试剂盒 ,英文名: ELISA Kit for Metaxin 3 (MTX3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14451 轴突生长诱向因子5(Ntn5)酶联免疫试剂盒 ,英文名: ELISA Kit for Netrin 5 (Ntn5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14452 假尿苷酸合酶3(PUS3)酶联免疫试剂盒 ,英文名: ELISA Kit for Pseudouridylate Synthase 3 (PUS3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14453 Anoctamin 10蛋白(ANO10)酶联免疫试剂盒 ,英文名: ELISA Kit for Anoctamin 10 (ANO10) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14454 Akirin 1蛋白(AKIRIN1)酶联免疫试剂盒 ,英文名: ELISA Kit for Akirin 1 (AKIRIN1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14455 Tectonic 2蛋白(TCTN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Tectonic 2 (TCTN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14456 N-α-乙酰转移酶40(NαA40)酶联免疫试剂盒 ,英文名: ELISA Kit for N-Alpha-Acetyltransferase 40 (NaA40) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14457 核仁蛋白10(NOL10)酶联免疫试剂盒 ,英文名: ELISA Kit for Nucleolar Protein 10 (NOL10) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14458 N-乙酰转移酶15(NAT15)酶联免疫试剂盒 ,英文名: ELISA Kit for N-Acetyltransferase 15 (NAT15) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14459 羰基还原酶4(CBR4)酶联免疫试剂盒 ,英文名: ELISA Kit for Carbonyl Reductase 4 (CBR4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14460 肌醇单磷酸酶3(IMPA3)酶联免疫试剂盒 ,英文名: ELISA Kit for Inositol Monophosphatase 3 (IMPA3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14461 假尿苷酸合酶7(PUS7)酶联免疫试剂盒 ,英文名: ELISA Kit for Pseudouridylate Synthase 7 (PUS7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14462 肌球蛋白ⅩⅨ(MYO19)酶联免疫试剂盒 ,英文名: ELISA Kit for Myosin XIX (MYO19) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14463 核仁蛋白9(NOL9)酶联免疫试剂盒 ,英文名: ELISA Kit for Nucleolar Protein 9 (NOL9) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14464 Raftlin 2蛋白(RFTN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Raftlin 2 (RFTN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14465 羧酸酯酶5A(CES5A)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxylesterase 5A (CES5A) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14466 腺苷酸激酶8(AK8)酶联免疫试剂盒 ,英文名: ELISA Kit for Adenylate Kinase 8 (AK8) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14467 筑丝蛋白5(TEKT5)酶联免疫试剂盒 ,英文名: ELISA Kit for Tektin 5 (TEKT5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14468 肌间蛋白3(MYOM3)酶联免疫试剂盒 ,英文名: ELISA Kit for Myomesin 3 (MYOM3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14469 羧酸酯酶4A(CES4A)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxylesterase 4A (CES4A) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14470 磷脂酶D5(PLD5)酶联免疫试剂盒 ,英文名: ELISA Kit for Phospholipase D5 (PLD5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14471 羟基丙酮酸异构酶(HYI)酶联免疫试剂盒 ,英文名: ELISA Kit for Hydroxypyruvate Isomerase (HYI) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14472 甘油磷酸胆碱磷酸二酯酶(GPCPD1)酶联免疫试剂盒 ,英文名: ELISA Kit for Glycerophosphocholine Phosphodiesterase (GPCPD1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14473 羧肽酶X2(CPX2)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase X2 (CPX2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14474 纺锤体蛋白4(SPIN4)酶联免疫试剂盒 ,英文名: ELISA Kit for Spindlin 4 (SPIN4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14475 纺锤体蛋白3(SPIN3)酶联免疫试剂盒 ,英文名: ELISA Kit for Spindlin 3 (SPIN3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14476 Fritz蛋白(FRTZ)酶联免疫试剂盒 ,英文名: ELISA Kit for Fritz (FRTZ) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14477 古梅斯蛋白2(AMZ2)酶联免疫试剂盒 ,英文名: ELISA Kit for Archaemetzincin 2 (AMZ2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14478 X-脯氨酰氨肽酶3(XPNPEP3)酶联免疫试剂盒 ,英文名: ELISA Kit for X-Prolyl Aminopeptidase 3 (XPNPEP3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14479 N-α-乙酰转移酶11(NαA11)酶联免疫试剂盒 ,英文名: ELISA Kit for N-Alpha-Acetyltransferase 11 (NaA11) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14480 皂素(Haponin)酶联免疫试剂盒 ,英文名: ELISA Kit for Haponin ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14481 孤儿素磷酸酶2(PHOSPHO2)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphatase, Orphan 2 (PHOSPHO2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14482 核仁蛋白12(NOL12)酶联免疫试剂盒 ,英文名: ELISA Kit for Nucleolar Protein 12 (NOL12) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14483 甘油激酶5(GK5)酶联免疫试剂盒 ,英文名: ELISA Kit for Glycerol Kinase 5 (GK5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14484 磷酸甘油酸变位酶5(PGAM5)酶联免疫试剂盒 ,英文名: ELISA Kit for Phosphoglycerate Mutase 5 (PGAM5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14485 93kDa核孔蛋白(NUP93)酶联免疫试剂盒 ,英文名: ELISA Kit for Nucleoporin 93kDa (NUP93) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14486 ATM交互子(ATMIN)酶联免疫试剂盒 ,英文名: ELISA Kit for ATM Interactor (ATMIN) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14487 Limkainβ1蛋白(LKAP)酶联免疫试剂盒 ,英文名: ELISA Kit for Limkain b1 (LKAP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14488 Marapsin 2蛋白(MPN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Marapsin 2 (MPN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14489 视黄醇脱氢酶15(RDH15)酶联免疫试剂盒 ,英文名: ELISA Kit for Retinol Dehydrogenase 16 (RDH16) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14490 Penguin蛋白(PEN)酶联免疫试剂盒 ,英文名: ELISA Kit for Penguin (PEN) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14491 Misato同源物1(MSTO1)酶联免疫试剂盒 ,英文名: ELISA Kit for Misato Homolog 1 (MSTO1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14492 腈水解酶2(NIT2)酶联免疫试剂盒 ,英文名: ELISA Kit for Nitrilase 2 (NIT2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14493 核仁蛋白58(NOP58)酶联免疫试剂盒 ,英文名: ELISA Kit for Nucleolar protein 58 (NOP58) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14494 仙人掌素(Cactin)酶联免疫试剂盒 ,英文名: ELISA Kit for Cactin ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14495 胰安肽(Aglycin)酶联免疫试剂盒 ,英文名: ELISA Kit for Aglycin ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14496 血管紧张素1-7(Ang1-7)酶联免疫试剂盒 ,英文名: ELISA Kit for Angiotensin 1-7 (Ang1-7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14497 透明质酸氨基葡糖苷酶1(HYAL1)酶联免疫试剂盒 ,英文名: ELISA Kit for Hyaluronoglucosaminidase 1 (HYAL1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14498 RPE脊椎蛋白(RPESP)酶联免疫试剂盒 ,英文名: ELISA Kit for RPE Spondin (RPESP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14499 Sarcosynapsin蛋白(SRYP)酶联免疫试剂盒 ,英文名: ELISA Kit for Sarcosynapsin (SRYP) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14500 Shisa同源物5(SHISA5)酶联免疫试剂盒 ,英文名: ELISA Kit for Shisa Homolog 5 (SHISA5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14501 分离蛋白2(SCRN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Secernin 2 (SCRN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14502 分离蛋白3(SCRN3)酶联免疫试剂盒 ,英文名: ELISA Kit for Secernin 3 (SCRN3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14503 信号素3G(SEMA3G)酶联免疫试剂盒 ,英文名: ELISA Kit for Semaphorin 3G (SEMA3G) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14504 磷脂酶D6(PLD6)酶联免疫试剂盒 ,英文名: ELISA Kit for Phospholipase D6 (PLD6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14505 Embigin蛋白(EMB)酶联免疫试剂盒 ,大鼠腹水癌细胞;Walker/LLC-WRC 256英文名: ELISA Kit for Embigin (EMB) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14506 核糖核酸外切酶2(ERI2)酶联免疫试剂盒 ,英文名: ELISA Kit for Exoribonuclease 2 (ERI2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A14507 筑丝蛋白4(TEKT4)酶联免疫试剂盒 ,英文名: ELISA Kit for Tektin 4 (TEKT4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
钙网蛋白3(CALR3)ELISA检测试剂盒
钙营养蛋白1(CALN1)ELISA检测试剂盒
钙周期素结合蛋白(CACYBP)ELISA检测试剂盒
盖膜蛋白α(TECTα)ELISA检测试剂盒
盖膜蛋白β(TECTβ)ELISA检测试剂盒
甘氨酸脒基转移酶(GATM)ELISA检测试剂盒
甘氨酸脱氢酶(GLDC)ELISA检测试剂盒
甘氨酸转运蛋白1(GLYT1)ELISA检测试剂盒
甘氨酸转运蛋白2(GLYT2)ELISA检测试剂盒
甘氨酰tRNA合成酶(GARS)ELISA检测试剂盒
甘胺酸-N-甲基转移酶(GNMT)ELISA检测试剂盒
甘丙肽(GAL)ELISA检测试剂盒
甘丙肽受体1(GALR1)ELISA检测试剂盒
甘丙肽受体2(GALR2)ELISA检测试剂盒
甘丙肽受体3(GALR3)ELISA检测试剂盒
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