供货周期: | 现货 |
品牌: | ATCC |
型号: | 1000000个细胞数/瓶 |
货号: | Cell-3806 |
CAS号: | 说明书有详尽的标注 |
人胚肺细胞VA13细胞;WI-38 VA13亚系2RA
成纤维细胞样
贴壁生长
该细胞系衍生自WI-38细胞。WI-38细胞感染低滴度的SV40病毒,经过连续传代、克隆选择获得多株亚系,其中2RA亚系含有SV40 neo (T)和转移抗原,从而改变其有限细胞系的特性,可在常规条件下连续传代。该细胞系的实验操作甚少在生物安全P2级条件下进行。
MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1:3传代,2-3天传一代
C265
基础培养基+5%DMSO+20%FBS
公司细胞的简洁信息:
(1)储存:液氮。
(2)运输:干冰。
(3)用途:只可用于科研。
(4)第二代培养末期液氮冻存。
(5)人胚肺细胞VA13细胞;WI-38 VA13亚系2RA每冻存管细胞数:>5×105 cells/1ml。
(6)不含有HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌。
收到人胚肺细胞VA13细胞;WI-38 VA13亚系2RA后的处理:
1)收到细胞后,首先观察瓶身是否完好(注意有无缝隙,裂痕)。
2)显微镜下观察细胞的生长状况,有无细胞漂浮。
3)用新洁尔灭消毒瓶口及瓶身。
4)打开瓶口,再次用新洁尔灭消毒瓶口(可能会有液体溢出,注意不要碰到液体),酒精灯烤瓶口消毒。
5)将培养液转移至50ml离心管或广口瓶中(若细胞漂浮严重,离心培养基,1000g*5分钟,将离心后的培养基转移至另一个大离心管中,留一点吹打细胞沉淀成悬液,回收细胞至原培养瓶),若漂浮不严重,亦离心一下。
6)在原培养瓶中留一部分原装培养液,再补加自己新配的培养基至适当容积,例如4ml,让细胞适应一下环境。 当细胞长到70-80%,冻存大部分,小部分传代。
A15280 木糖基转移酶Ⅰ(XYLT1)酶联免疫试剂盒 ,英文名: ELISA Kit for Xylosyltransferase I (XYLT1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15281 输出蛋白7(XPO7)酶联免疫试剂盒 ,英文名: ELISA Kit for Exportin 7 (XPO7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15282 输出蛋白6(XPO6)酶联免疫试剂盒 ,英文名: ELISA Kit for Exportin 6 (XPO6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15283 输出蛋白4(XPO4)酶联免疫试剂盒 ,英文名: ELISA Kit for Exportin 4 (XPO4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15284 输出蛋白3(XPO3)酶联免疫试剂盒 ,英文名: ELISA Kit for Exportin 3 (XPO3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15285 24-脱氢胆固醇还原酶(DHCR24)酶联免疫试剂盒 ,英文名: ELISA Kit for 24-Dehydrocholesterol Reductase (DHCR24) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15286 蛋白O-岩藻糖基转移酶2(POFUT2)酶联免疫试剂盒 ,英文名: ELISA Kit for Protein O-Fucosyltransferase 2 (POFUT2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15287 岩藻糖基转移酶2(FUT2)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 2 (FUT2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15288 岩藻糖基转移酶1(FUT1)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 1 (FUT1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15289 岩藻糖基转移酶11(FUT11)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 11 (FUT11) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15290 岩藻糖基转移酶5(FUT5)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 5 (FUT5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15291 岩藻糖基转移酶6(FUT6)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 6 (FUT6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15292 岩藻糖基转移酶7(FUT7)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 7 (FUT7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15293 岩藻糖基转移酶8(FUT8)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 8 (FUT8) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15294 岩藻糖基转移酶9(FUT9)酶联免疫试剂盒 ,英文名: ELISA Kit for Fucosyltransferase 9 (FUT9) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15295 氨基酰化酶2(ACY2)酶联免疫试剂盒 ,英文名: ELISA Kit for Aminoacylase 2 (ACY2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15296 内收蛋白3(ADD3)酶联免疫试剂盒 ,英文名: ELISA Kit for Adducin 3 (ADD3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15297 内收蛋白2(ADD2)酶联免疫试剂盒 ,英文名: ELISA Kit for Adducin 2 (ADD2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15298 腺苷酸激酶3(AK3)酶联免疫试剂盒 ,英文名: ELISA Kit for Adenylate Kinase 3 (AK3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15299 腺苷酸激酶4(AK4)酶联免疫试剂盒 ,英文名: ELISA Kit for Adenylate Kinase 4 (AK4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15300 腺苷酸激酶1(AK1)酶联免疫试剂盒 ,英文名: ELISA Kit for Adenylate Kinase 1 (AK1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15301 外核苷酸焦磷酸酶/磷酸二酯酶3(ENPP3)酶联免疫试剂盒 ,英文名: ELISA Kit for Ectonucleotide Pyrophosphatase/Phosphodiesterase 3 (ENPP3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15302 碱核蛋白2(BNC2)酶联免疫试剂盒 ,英文名: ELISA Kit for Basonuclin 2 (BNC2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15303 布皮质素4(BEST4)酶联免疫试剂盒 ,英文名: ELISA Kit for Bestrophin 4 (BEST4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15304 布皮质素3(BEST3)酶联免疫试剂盒 ,英文名: ELISA Kit for Bestrophin 3 (BEST3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15305 布皮质素2(BEST2)酶联免疫试剂盒 ,英文名: ELISA Kit for Bestrophin 2 (BEST2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15306 钙蛋白酶6(CAPN6)酶联免疫试剂盒 ,英文名: ELISA Kit for Calpain 6 (CAPN6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15307 钙蛋白酶7(CAPN7)酶联免疫试剂盒 ,英文名: ELISA Kit for Calpain 7 (CAPN7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15308 钙蛋白酶11(CAPN11)酶联免疫试剂盒 ,英文名: ELISA Kit for Calpain 11 (CAPN11) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15309 钙磷蛋白2(CAPS2)酶联免疫试剂盒 ,英文名: ELISA Kit for Calcyphosine 2 (CAPS2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15310 羰基还原酶3(CBR3)酶联免疫试剂盒 ,英文名: ELISA Kit for Carbonyl Reductase 3 (CBR3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15311 中心体蛋白3(CETN3)酶联免疫试剂盒 ,英文名: ELISA Kit for Centrin 3 (CETN3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15312 中心体蛋白2(CETN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Centrin 2 (CETN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15313 中心体蛋白1(CETN1)酶联免疫试剂盒 ,英文名: ELISA Kit for Centrin 1 (CETN1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15314 羧酸酯酶2(CES2)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxylesterase 2 (CES2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15315 羧酸酯酶3(CES3)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxylesterase 3 (CES3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15316 糖合酶3(CHSY3)酶联免疫试剂盒 ,英文名: ELISA Kit for Carbohydrate Synthase 3 (CHSY3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15317 封闭蛋白2(CLDN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 2 (CLDN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15318 封闭蛋白5(CLDN5)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 5 (CLDN5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15319 封闭蛋白16(CLDN16)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 16 (CLDN16) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15320 封闭蛋白7(CLDN7)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 7 (CLDN7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15321 封闭蛋白8(CLDN8)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 8 (CLDN8) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15322 封闭蛋白12(CLDN12)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 12 (CLDN12) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15323 封闭蛋白14(CLDN14)酶联免疫试剂盒 ,英文名: ELISA Kit for Claudin 14 (CLDN14) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15324 Archain 1蛋白(ARCN1)酶联免疫试剂盒 ,英文名: ELISA Kit for Archain 1 (ARCN1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15325 羧肽酶A4(CPA4)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase A4 (CPA4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15326 羧肽酶A5(CPA5)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase A5 (CPA5) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15327 羧肽酶A6(CPA6)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase A6 (CPA6) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15328 羧肽酶D(CPD)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase D (CPD) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15329 羧肽酶N2(CPN2)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase N2 (CPN2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15330 羧肽酶O(CPO)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase O (CPO) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15331 羧肽酶Z(CPZ)酶联免疫试剂盒 ,英文名: ELISA Kit for Carboxypeptidase Z (CPZ) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15332 丝氨酸羧肽酶1(SCPEP1)酶联免疫试剂盒 ,英文名: ELISA Kit for Serine Carboxypeptidase 1 (SCPEP1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15333 复合素3(CPLX3)酶联免疫试剂盒 ,英文名: ELISA Kit for Complexin 3 (CPLX3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15334 复合素4(CPLX4)酶联免疫试剂盒 ,英文名: ELISA Kit for Complexin 4 (CPLX4) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15335 隐花色素2(CRY2)酶联免疫试剂盒 ,英文名: ELISA Kit for Cryptochrome 2 (CRY2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15336 晶状体蛋白λ1(CRYλ1)酶联免疫试剂盒 ,英文名: ELISA Kit for Crystallin Lambda 1 (CRYl1) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15337 晶状体蛋白μ(CRYμ)酶联免疫试剂盒 ,英文名: ELISA Kit for Crystallin Mu (CRYm) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15338 晶状体蛋白ζ(CRYζ)酶联免疫试剂盒 ,英文名: ELISA Kit for Crystallin Zeta (CRYz) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15339 Cullin 9蛋白(CUL9)酶联免疫试剂盒 ,英文名: ELISA Kit for Cullin 9 (CUL9) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15340 Cullin 7蛋白(CUL7)酶联免疫试剂盒 ,英文名: ELISA Kit for Cullin 7 (CUL7) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15341 Cullin 2蛋白(CUL2)酶联免疫试剂盒 ,人胚肺细胞VA13细胞;WI-38 VA13亚系2RA英文名: ELISA Kit for Cullin 2 (CUL2) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15342 Cullin 3蛋白(CUL3)酶联免疫试剂盒 ,英文名: ELISA Kit for Cullin 3 (CUL3) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
A15343 Cullin 4A蛋白(CUL4A)酶联免疫试剂盒 ,英文名: ELISA Kit for Cullin 4A (CUL4A) ,适用种属: Homo sapiens (Human) ,规格: 48T/96T
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