供货周期: | 一周 |
品牌: | EterLife |
规格: | 0.1ml/100μg /0.2ml/200μg |
货号: | TE-KT-0136 |
CAS号: | 详见产品说明书 |
磷酸化自噬相关蛋白4A抗体
〖别名〗AI627006; Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A; MGC107179.
〖浓度〗1mg/1ml
〖规格〗磷酸化自噬相关蛋白4A抗体0.1ml/100μg
〖抗体来源〗Rabbit
〖克隆类型〗polyclonal
〖交叉反应〗Human
〖产品类型〗一抗 磷酸化抗体
〖研究领域〗肿瘤 心血管 细胞生物 免疫学 信号转导 细胞凋亡 线粒体 细胞外基质 泛素
〖蛋白分子量〗predicted molecular weight: 45kDa
〖性状〗Lyophilized or Liquid
〖免疫原〗KLH conjugated Synthesised phosphopeptide derived from human ATG4A around the phosphorylation site of Ser100
〖亚型〗IgG
〖纯化方法〗affinity purified by Protein A
〖储存液〗0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
〖产品应用〗WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
〖保存条件〗Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
〖产品介绍〗ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Function : Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.
Subcellular Location : Cytoplasm (Probable).
Tissue Specificity : Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver.
Similarity : Belongs to the peptidase C54 family.
Database links : UniProtKB/Swiss-Prot: Q8WYN0.1
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