供货周期: | 一周 |
品牌: | EterLife |
规格: | 0.1ml/100μg 、0.2ml/200μg |
货号: | TE-KT-5103 |
CAS号: | 详见产品说明书 |
异质性核糖核蛋白C1/C2抗体
〖别名〗Heterogeneous nuclear ribonucleoprotein C; Heterogeneous nuclear ribonucleoproteins C1/C2; HNRNP; hnRNP C1 / hnRNP C2; hnRNP C1/C2; hnRNPC; HNRPC; HNRPC_HUMAN; Nuclear ribonucleoprotein particle C1 protein; Nuclear ribonucleoprotein particle C2 protein; SNRPC.
〖浓度〗1mg/1ml
〖规格〗0.2ml/200μg
〖抗体来源〗Rabbit
〖克隆类型〗polyclonal
〖交叉反应〗异质性核糖核蛋白C1/C2抗体Human, Mouse, Rat, Dog, Pig, Horse, Rabbit
〖产品类型〗一抗
〖研究领域〗细胞生物 免疫学 表观遗传学
〖蛋白分子量〗predicted molecular weight: 34kDa
〖性状〗Lyophilized or Liquid
〖免疫原〗KLH conjugated synthetic peptide derived from human HNRPC
〖亚型〗IgG
〖纯化方法〗affinity purified by Protein A
〖储存液〗Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
〖产品应用〗WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
〖保存条件〗Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
〖产品介绍〗Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.
Function : UniProtKB/Swiss-Prot: P07910.4
Subunit : Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1.
Subcellular Location : Nucleus. Component of ribonucleosomes.
Post-translational modifications : Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.
Sumoylated. Sumoylation reduces affinity for mRNA.
Similarity : Belongs to the RRM HNRPC family. RALY subfamily.
Contains 1 RRM (RNA recognition motif) domain.
Database links : UniProtKB/Swiss-Prot: P07910.4
做过实验的亲们都知道异质性核糖核蛋白C1/C2抗体反应规律,仔细观察我们会发现当抗原第一次进入机体时,需经一定的潜伏期才能产生抗体,且抗体产生的量也不多,在体内维持的时间也较短。当相同抗原第二次进入机体后,开始时,由于原有抗体中的一部分与再次进入的抗原结合,可使原有抗体量略为降低。随后,抗体效价迅速大量增加,可比初次反应产生的多几倍到几十倍,在体内留存的时间亦较长。因此得出由抗原刺激机体产生的抗体,经过一定时间后可逐渐消失。此时若再次接触抗原,可使已消失的抗体快速上升。如再次刺激机体的抗原与初次相同,则称为特异性回忆反应;若与初次反应不同,则称为非特异性回忆反应。
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Ab-(010)-16773 Anti-ADAM-TS7/FITC 荧光素标记整合素样金属蛋白酶与凝血酶1型-7抗体IgG ,规格: 0.2ml ,英文名: Anti-ADAM-TS7/FITC
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Ab-(010)-16787 Anti-Acinus L/S/FITC 荧光素标记腺泡Acinus L/S抗体IgG ,规格: 0.2ml ,英文名: Anti-Acinus
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Ab-(010)-16797 Anti-Rat IgA/FITC 荧光素标记兔抗大鼠IgA抗体 ,规格: 0.2ml ,英文名: Anti-Rat
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