大鼠抗酒石酸酸性磷酸酶5b(TRACP5b)检测试剂盒

2015-09-07 19:25  下载量:1

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The kit assay Rat TRACP5b level in the sample, use Purified Rat TRACP5b antibody to coat microtiter plate wells, make solid-phase antibody, then add TRACP5b to wells, Combined TRACP5b antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat TRACP5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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