大鼠精脒精胺N1-乙酰基转移酶1(SAT1)ELISA试剂盒

2015-09-10 16:04  下载量:2

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Rat SAT1 FOR RESEARCH USE ONLY Assay range:1.5ng/L - 48ng/L 96 determinations Purpose This kit allows for the determination of SAT1 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat SAT1 level in the sample, use Purified Rat SAT1 antibody to coat microtiter plate wells, make solid-phase antibody, then add SAT1 to wells, Combined SAT1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat SAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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