大鼠抗酒石酸酸性磷酸酶5b(TRACP5b)ELISA试剂盒

2015-09-10 16:05 下载量：2

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Rat tartrate-resistant acid phosphatase 5b（TRACP5b） FOR RESEARCH USE ONLY Assay range：60pg/mL - 1600pg/mL 96 determinations Purpose This kit allows for the determination of TRACP5b concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat TRACP5b level in the sample, use Purified Rat TRACP5b antibody to coat microtiter plate wells, make solid-phase antibody, then add TRACP5b to wells, Combined TRACP5b antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat TRACP5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物技术有限公司

银牌会员 | 第10年

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