大鼠血管紧张素I（AngI)ELISA试剂盒

2015-09-10 16:09 下载量：2

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Rat Ang I FOR RESEARCH USE ONLY Assay range：50pg/ml- 2600pg/ml 96 determinations Purpose This kit allows for the determination of ANG I concentration in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat ANG I level in the sample，use Purified Rat ANG I antibody to coat microtiter plate wells, make solid-phase antibody, then add ANG I to wells, Combined ANG I antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat ANG I in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海信裕生物技术有限公司

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