人上皮细胞粘附分子(Ep-CAMCD362)ELISA试剂盒

Human Ep-CAM/CD362 FOR RESEARCH USE ONLY Assay range：1.2ng/L -45ng/L 96 determinations Purpose This kit allows for the determination of Ep-CAM/CD362 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human Ep-CAM/CD362 level in the sample, use Purified Human Ep-CAM/CD362 antibody to coat microtiter plate wells, make solid-phase antibody, then add Ep-CAM/CD362 to wells, Combined Ep-CAM/CD362 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Ep-CAM/CD362 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

文献贡献者

上海信裕生物技术有限公司

银牌会员 | 第10年

查看全部资料