供货周期: | 现货 |
品牌: | IBL-America |
规格: | 96T/48T |
货号: | xy-E10130 |
CAS号: |
人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)检测试剂盒实验操作常见问题分析
异常 | 结果描述 | 原因分析 | 对策 |
白板 | 显色步骤结束后,酶标板所有孔均无颜色。阳性对照不显色。 | 试剂已过效期,或不同试剂盒组分混用 | 检查试剂的组分及批号,确认未过期以及所有组分均属对应的试剂盒。不同试剂盒或不同批号的试剂不能混用。 |
错加、漏加试剂底物、显色剂 A或B | 严格按说明书的步骤操作,加完试剂后可观察一下液面高度是否一致,以减少漏加现象。 | ||
洗板及加样过程中,酶标受污染失活失去催化显色剂显色的能力 | 确认盛酶标的容器不含酶抑制剂如叠氮钠等,确认配制洗液的容器已洗干净。 | ||
终止液误作洗涤液稀释或当底物缓冲液配制 | 每次配制时人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)检测试剂盒都应看清标签标明物质 | ||
蒸馏水有问题 | 确认配制洗液的纯化水达到要求且未污染,与好蒸馏水比较。 |
标本及采集、贮运因素:严重溶血 ,以 HRP 为标记的ELISA 测定中,残留在孔内的血红蛋白具有过氧化物酶样活性,催化底物显色造成假阳性; 混有红细胞的血清 易沉淀或附着在聚乙烯孔内不易洗净;如有 细菌污染 ,菌体中可能含有内源性HRP,也会产生假阳性反应; 标本凝固不全 ,有时为了争取时间快速检测,常在血液还未开始凝固时即强行离心分离血清,使血清中仍残留部分纤维蛋白原,在ELISA测定过程中可以形成肉眼可见的纤维蛋白块,易造成假阳性结果; 采血试管洗涤不彻底 、反复使用易交叉污染; 塑料试管能吸附抗原物质 ,样本久置在塑料管内会使样本内抗原含量下降造成假阴性。
人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)检测试剂盒其它相关试剂盒:
xyP862Hu人粘蛋白12(MUC12)ELISA试剂盒ELISA Kit for Mucin 12, HZll SurfaHZ Associated (MUC12)
xyQ641Hu人棘皮动物微管关联蛋白样蛋白2(EML2)ELISA试剂盒ELISA Kit for Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)
xyQ741Hu人胎盘特异性蛋白9(PLAC9)ELISA试剂盒ELISA Kit for PlaHZnta Specific Protein 9 (PLAC9)
xyT372Hu人短腭肺鼻腔上皮癌关联蛋白2(SPLUNC2)ELISA试剂盒ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2)
xyT373Hu人短腭肺鼻腔上皮癌关联蛋白3(SPLUNC3)ELISA试剂盒ELISA Kit for Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3)
xyA596Hu人铜伴侣超氧化物歧化酶(SOD4)ELISA试剂盒ELISA Kit for Superoxide Dismutase, Copper Chaperone (SOD4)
xyA751Hu人细胞程序性死亡蛋白1(PDCD1)ELISA试剂盒ELISA Kit for Programmed HZll Death Protein 1 (PDCD1)
xyA755Hu人肌球蛋白重链2(MYH2)ELISA试剂盒ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)
xyD321Hu人20S-蛋白酶体(20S-PSM)ELISA试剂盒ELISA Kit for 20S-Proteasome (20S-PSM) 人肿瘤坏死因子相关凋亡诱导配体4(TRAIL-R4)检测试剂盒
xyD329Hu人蛋白酶体亚基α1(PSMα1)ELISA试剂盒ELISA Kit for Proteasome Subunit Alpha Type 1 (PSMa1)
xyD416Hu人肌球蛋白重链4(MYH4)ELISA试剂盒ELISA Kit for Myosin Heavy Chain 4, Skeletal Muscle (MYH4)
xyD418Hu人肌球蛋白重链7(MYH7)ELISA试剂盒ELISA Kit for Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)
xyD419Hu人肌球蛋白重链6(MYH6)ELISA试剂盒ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
xyD486Hu人5-羟色胺受体2A(HTR2A)ELISA试剂盒ELISA Kit for 5-Hydroxytryptamine ReHZptor 2A (HTR2A)
xyD934Hu人肿瘤坏死因子配体超家族成员9(TNFSF9)ELISA试剂盒ELISA Kit for Tumor Necrosis Factor Ligand Superfamily, Member 9 (TNFSF9)
xyD964Hu人核受体相关蛋白1(NURR1)ELISA试剂盒ELISA Kit for Nuclear ReHZptor Related Protein 1 (NURR1)
xyE478Hu人睾丸阴离子转运蛋白1(TAT1)ELISA试剂盒ELISA Kit for Testis Anion Transporter 1 (TAT1)
xyE801Hu人AMPA离子能谷氨酸受体1(GRIA1)ELISA试剂盒ELISA Kit for Glutamate ReHZptor, Ionotropic, AMPA 1 (GRIA1)
xyE802Hu人AMPA离子能谷氨酸受体2(GRIA2)ELISA试剂盒ELISA Kit for Glutamate ReHZptor, Ionotropic, AMPA 2 (GRIA2)
xyE804Hu人AMPA离子能谷氨酸受体4(GRIA4)ELISA试剂盒ELISA Kit for Glutamate ReHZptor, Ionotropic, AMPA 4 (GRIA4)
xyF557Hu人线粒体解偶联蛋白1(UCP1)ELISA试剂盒ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)
xyF824Hu人易位关联膜蛋白1(TRAM1)ELISA试剂盒ELISA Kit for Translocation Associated Membrane Protein 1 (TRAM1)
xyG301Hu人黑素瘤缺乏因子1(AIM1)ELISA试剂盒ELISA Kit for Absent In Melanoma 1 (AIM1)
xyG753Hu人TNF受体关联因子3(TRAF3)ELISA试剂盒ELISA Kit for TNF ReHZptor Associated Factor 3 (TRAF3)
xyG755Hu人TNF受体关联因子5(TRAF5)ELISA试剂盒ELISA Kit for TNF ReHZptor Associated Factor 5 (TRAF5)
xyH022Hu人Toll样受体衔接分子1(TICAM1)ELISA试剂盒ELISA Kit for Toll Like ReHZptor Adaptor Molecule 1 (TICAM1)
xyH061Hu人TANK结合激酶1(TBK1)ELISA试剂盒ELISA Kit for TANK Binding Kinase 1 (TBK1)
xyH596Hu人V-Maf肌肉腱膜纤维肉瘤癌基因同源物(MAF)ELISA试剂盒ELISA Kit for V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF)
相关产品
小鼠精氨酸加压素受体1B(AVPR1B)检测试剂盒
小鼠心肌肌钙蛋白T(TNNT2)检测试剂盒
小鼠谷氨酰胺酶2(GLS2)检测试剂盒
小鼠谷氨酰胺酶(GLS)检测试剂盒
小鼠胸肾表达趋化因子(BRAK)检测试剂盒
小鼠激肽原1(KNG1)检测试剂盒
小鼠VGF神经生长因子诱导蛋白(VGF)检测试剂盒
小鼠肝配蛋白B2(EFNB2)检测试剂盒
小鼠尿调蛋白(UMOD)检测试剂盒
小鼠胆碱乙酰转移酶(ChAT)检测试剂盒
小鼠脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒
小鼠YY肽(PYY)检测试剂盒
小鼠N-乙酰半乳糖胺酶α(NAGα)检测试剂盒
小鼠信号素7A(SEMA7A)检测试剂盒
小鼠CD83分子(CD83)检测试剂盒
详细地址
关注
拨打电话
留言咨询