人干扰素γ(IFNγ)ELISA试剂盒

人干扰素γ(IFNγ)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人干扰素γ(IFNγ)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人干扰素γ(IFNγ)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人干扰素γ(IFNγ)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人干扰素γ(IFNγ)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H0437c 人抗甲状腺过氧化物酶抗体(TPO-Ab)ELISA试剂盒   Human TPO-Ab (Anti-Thyroid-Peroxidase Antibody) ELISA Kit

XY-EL-H0438c 人胰岛素样生长因子2-mRNA结合蛋白3(IGF2BP3)ELISA试剂盒 Human IGF2BP3 (Insulin Like Growth Factor 2 mRNA Binding Protein 3) ELISA Kit 

XY-EL-H0439c 人肌联蛋白(TTN)ELISA试剂盒 Human TTN (Titin) ELISA Kit

XY-EL-H0440c 人胰岛素样生长因子2受体(IGF2R)ELISA试剂盒 Human IGF2R (Insulin Like Growth Factor 2 Receptor) ELISA Kit

XY-EL-H0441c 人胰岛素样生长因子酸不稳定亚基(IGFALS)ELISA试剂盒 Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) ELISA Kit 

XY-EL-H0442c 人胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒 Human IGFBP-1 (Insulin Like Growth Factor Binding Protein 1) ELISA Kit

XY-EL-H0443c 人胰岛素样生长因子结合蛋白5(IGFBP-5)ELISA试剂盒 Human IGFBP-5 (Insulin Like Growth Factor Binding Protein 5) ELISA Kit

XY-EL-H0444c 人胰岛素样生长因子结合蛋白4(IGFBP-4)ELISA试剂盒 Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit

XY-EL-H0445c 人抗II型胶原抗体(Anti-CIIAb)ELISA试剂盒 Human Anti-CIIAb (Anti-Type II Collagen Antibody) ELISA Kit  

XY-EL-H0446c 人胰岛素样生长因子结合蛋白2(IGFBP-2)ELISA试剂盒 Human IGFBP-2 (Insulin Like Growth Factor Binding Protein 2) ELISA Kit

XY-EL-H0447c 人胰岛素样生长因子结合蛋白7(IGFBP-7)ELISA试剂盒 Human IGFBP-7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit

XY-EL-H0448c 人膜联蛋白A2(ANXA2 )ELISA试剂盒   Human ANXA2 (Annexin A2) ELISA Kit

XY-EL-H0450c 人胰岛素样生长因子结合蛋白6(IGFBP-6)ELISA试剂盒 Human IGFBP-6 (Insulin Like Growth Factor Binding Protein 6) ELISA Kit

XY-EL-H0452c 人胰岛素受体(INSR)ELISA试剂盒 Human INSR (Insulin Receptor) ELISA Kit

XY-EL-H0453c 人活化蛋白C(APC)ELISA试剂盒 Human APC (Activated Protein C) ELISA Kit

XY-EL-H0456c 人Apelin蛋白(APLN)ELISA试剂盒 Human APLN (Apelin) ELISA Kit

XY-EL-H0458c 人Apelin 13蛋白(AP13)ELISA试剂盒   Human AP13 (Apelin 13) ELISA Kit

XY-EL-H0459c 人Apelin 36蛋白(AP36)ELISA试剂盒 Human AP36 (Apelin 36) ELISA Kit

XY-EL-H0460c 人抗载脂蛋白A1抗体(Apo A1-Ab)ELISA试剂盒   Human Apo A1-Ab (Anti-Apolipoprotein A1 Antibody) ELISA Kit

XY-EL-H0461c 人脂质运载蛋白1(LCN1)ELISA试剂盒 Human LCN1 (Lipocalin 1) ELISA Kit

XY-EL-H0462c 人载脂蛋白A2(ApoA2)ELISA试剂盒 Human ApoA2 (Apolipoprotein A2) ELISA Kit

XY-EL-H0463c 人载脂蛋白A4(ApoA4)ELISA试剂盒 Human ApoA4 (Apolipoprotein A4) ELISA Kit

XY-EL-H0464c 人载脂蛋白B(ApoB)ELISA试剂盒 Human ApoB (Apolipoprotein B) ELISA Kit 

XY-EL-H0465c 人载脂蛋白C1(ApoC1)ELISA试剂盒 Human ApoC1 (Apolipoprotein C1) ELISA Kit

XY-EL-H0466c 人载脂蛋白C2(ApoC2)ELISA试剂盒 Human ApoC2 (Apolipoprotein C2) ELISA Kit

XY-EL-H0467c 人载脂蛋白C3(ApoC3)ELISA试剂盒 Human ApoC3 (Apolipoprotein C3) ELISA Kit

XY-EL-H0468c 人载脂蛋白C4(ApoC4)ELISA试剂盒 Human ApoC4 (Apolipoprotein C4) ELISA Kit

XY-EL-H0469c 人载脂蛋白D(ApoD)ELISA试剂盒 Human ApoD (Apolipoprotein D) ELISA Kit 

XY-EL-H0470c 人载脂蛋白E(ApoE)ELISA试剂盒 Human ApoE (Apolipoprotein E) ELISA Kit 

XY-EL-H0471c 人载脂蛋白F(ApoF)ELISA试剂盒 Human ApoF (Apolipoprotein F) ELISA Kit 

XY-EL-H0472c 人载脂蛋白H(ApoH)ELISA试剂盒   Human ApoH (Apolipoprotein H) ELISA Kit 

XY-EL-H0473c 人载脂蛋白M(ApoM)ELISA试剂盒   Human ApoM (Apolipoprotein M) ELISA Kit 

XY-EL-H0474c 人碳酸酐酶VI(CA6)ELISA试剂盒 Human CA6 (Carbonic Anhydrase VI) ELISA Kit

XY-EL-H0475c 人载脂蛋白A5(ApoA5)ELISA试剂盒   Human ApoA5 (Apolipoprotein A5) ELISA Kit