人补体因子H(CFH)ELISA试剂盒

人补体因子H(CFH)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人补体因子H(CFH)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人补体因子H(CFH)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人补体因子H(CFH)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人补体因子H(CFH)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-P0927c 猪胱天蛋白酶14(CASP14)ELISA试剂盒 Porcine CASP14 (Caspase 14) ELISA Kit

XY-EL-P0928c 猪松弛素/胰岛素样肽受体1(RXFP1)ELISA试剂盒 Porcine RXFP1 (Relaxin/Insulin Like Family Peptide Receptor 1) ELISA Kit 

XY-EL-P0931c 猪血小板膜糖蛋白II b/III a(GPIIb/IIIa)ELISA试剂盒 Porcine GPIIb/IIIa (Platelet Membrane Glycoprotein IIb/IIIa) ELISA Kit

XY-EL-P0932c 猪多聚免疫球蛋白受体(PIGR/SC)ELISA试剂盒 Porcine PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) ELISA Kit

XY-EL-P0934c 猪α1连接素(CTNNα1)ELISA试剂盒 Porcine CTNNα1 (Catenin, Alpha 1) ELISA Kit 

XY-EL-P0935c 猪嗜酸粒细胞趋化因子受体(ECFR)ELISA试剂盒 Porcine ECFR (Eosinophil Chemotactic Factor Receptor) ELISA Kit

XY-EL-P0936c 猪粘膜相关上皮趋化因子(MEC/CCL28)ELISA试剂盒 Porcine MEC/CCL28 (Mucosae Associated Epithelia Chemokine) ELISA Kit

XY-EL-P0938c 猪白血病抑制因子受体(LIFR)ELISA试剂盒 Porcine LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit 

XY-EL-P0940c 猪晶体蛋白(CLC)ELISA试剂盒 Porcine CLC (Charcot Leyden Crystal Protein) ELISA Kit

XY-EL-P0941c 猪髓鞘相关糖蛋白(MAG)ELISA试剂盒   Porcine MAG (Myelin Associated Glycoprotein) ELISA Kit

XY-EL-P0942c 猪11去氢血栓烷B2(11-DH-TXB2)ELISA试剂盒 Porcine 11-DH-TXB2 (11-Dehydro-thromboxane B2) ELISA Kit

XY-EL-P0943c 猪抗角蛋白丝聚集素/丝集蛋白抗体(AFA)ELISA试剂盒 Porcine AFA (Anti-Filaggrin Antibody) ELISA Kit

XY-EL-P0947c 猪FMS样酪氨酸激酶3(Flt3)ELISA试剂盒 Porcine Flt3 (FMS Like Tyrosine Kinase 3) ELISA Kit

XY-EL-P0948c 猪前胶原C端肽酶(PCP)ELISA试剂盒 Porcine PCP (Procollagen C Peptidase) ELISA Kit

XY-EL-P0949c 猪α羟基丁酸脱氢酶(αHBDH)ELISA试剂盒 Porcine αHBDH (α-Hydroxybutyrate Dehydrogenase) ELISA Kit

XY-EL-P0950c 猪毒蕈碱型乙酰胆碱受体亚型M2(M-AChRM2)ELISA试剂盒 Porcine M-AChR M2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

XY-EL-P0951c 猪碳酸酐酶VB(CA5B)ELISA试剂盒 Porcine CA5B (Carbonic Anhydrase VB, Mitochondrial) ELISA Kit

XY-EL-P0952c 猪P62抗体(AP62A)ELISA试剂盒 Porcine AP62A (Anti-P62 Antibody)  ELISA Kit 

XY-EL-P0953c 猪着丝粒蛋白I(CENPI)ELISA试剂盒 Porcine CENPI (Centromere Protein I) ELISA Kit 

XY-EL-P0954c 猪核糖体蛋白L10(RPL10)ELISA试剂盒 Porcine RPL10 (Ribosomal Protein L10) ELISA Kit 

XY-EL-P0955c 猪葡萄糖-6-磷酸脱氢酶(G6PD)ELISA试剂盒 Porcine G6PD (Glucose 6 Phosphate Dehydrogenase) ELISA Kit 

XY-EL-P0956c 猪亚甲基四氢叶酸还原酶(MTHFR)ELISA试剂盒 Porcine MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

XY-EL-P0957c 猪三叶因子2(TFF2)ELISA试剂盒 Porcine TFF2 (Trefoil Factor 2) ELISA Kit 

XY-EL-P0959c 猪性别决定区Y框蛋白3(SOX3)ELISA试剂盒 Porcine SOX3 (Sex Determining Region Y Box Protein 3) ELISA Kit 

XY-EL-P0960c 猪补体成分2(C2)ELISA试剂盒   Porcine C2 (Complement Component 2) ELISA Kit 

XY-EL-P0961c 猪免疫球蛋白G Fc段低亲和力受体IIIa(FcγR3A)ELISA试剂盒 Porcine FcγR3A (Fc Fragment of IgG Low Affinity IIIa Receptor) ELISA Kit

XY-EL-P0962c 猪过氧化物酶体增殖物激活受体α(PPAR-α)ELISA试剂盒 Porcine PPAR-α (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit

XY-EL-P0964c 猪信号传导转录激活因子3(STAT3)ELISA试剂盒 Porcine STAT3 (Signal Transducer and Activator of Transcription 3) ELISA Kit 

XY-EL-P0965c 猪B细胞κ 轻肽基因增强子核因子抑制因子ε(Nfkbie)ELISA试剂盒 Porcine NFKBIE (Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor Epsilon) ELISA Kit

XY-EL-P0967c 猪尿素酶相关蛋白C(UreC)ELISA试剂盒 Porcine UreC (Urease Related Protein C) ELISA Kit

XY-EL-P0968c 猪N端中段骨钙素(N-MID-OT)ELISA试剂盒 Porcine N-MID-OT (N-MID Osteocalcin) ELISA Kit