人穿孔素1(PRF1)ELISA试剂盒

产品名称：人穿孔素1(PRF1)ELISA试剂盒

英文名称：ELISA Kit for Perforin 1 (PRF1)

大鼠酶联免疫试剂盒

规格：48孔配置/96孔配置

有效期：6个月

用途：本试剂盒仅供科学研究实验使用，不能用于医学诊断或其它用途

人穿孔素1(PRF1)ELISA试剂盒包被原的性质很重要，蛋白浓度，是否降解，这关系到你做出的抗体可不可以被其识别，所以保存抗原很重要，我做重组蛋白时，师兄都严格警告我一定要在冰浴下缓慢融化就是这个道理。人穿孔素1(PRF1)ELISA试剂盒还有有的包被原可能不是蛋白，对于生物素和脂类物质或小分子物质我们要事先对其改造再加以包被，我把方法简要的列出如下:①亲和素生物素:先亲和素先包被载体，加入生物素化的DNA，这种包被方法均匀、牢固，已扩大应用于各种抗原物质的定量测定。②脂类物质:可将其在有机溶剂(例如乙醇)中溶解后加入人穿孔素1(PRF1)ELISA试剂盒板孔中，开盖置冰箱过夜或冷风吹干，待酒精挥发后，让脂质自然干固在固相表面。③小分子必须依靠和大的蛋白载体偶联后才能固定在固相载体上。

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