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乙酸【AK法】检测试剂盒

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产品详情

产品货号：ZZK-K-ACETAK

产品品名：乙酸【AK法】检测试剂盒

英文品名：ACETIC ACID(ACETATE KINASE FORMAT) assay Kit

规格型号：550次（170.5 ml）

Analyser format for the specific assay of acetic acid (acetate) inbeverages and food products. On calibration, the prepared reagent islinear to > 28 micrograms of acetic acid per mL of assay solution.

INTRODUCTION:

The most widely used method for enzymatic quantification of acetic

acid is that employing acetyl-coenzyme A synthetase (ACS). However,

this method is based on the use of an indicator reaction catalysed by

L-malate dehydrogenase that is in permanent equilibrium, and therefore

a non-stoichiometric increase in absorbance is observed from the

acetate present in the sample. Thus, a slightly non-linear response

to increasing acetate concentration is observed upon calibration,

resulting in poor R2 values. In addition (depending on the supplier)

reagents prepared for auto-analyser applications can have very limited

on-machine stability, due to rapidly increasing blank absorbance values.

To overcome these issues, Megazyme developed this alternative acetic

acid kit, based on the enzyme acetate kinase (AK; see equations 1-3

below), especially for the auto-analyser user. This reagent has improved

on-machine stability, gives excellent linear calibration curves, and results

in a stoichiometric change in absorbance due to the acetic acid present

in the sample.

KITS:

Kits suitable for performing a minimum of 550 assays in auto-analyser

format are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Buffer (11 mL, pH 7.4) plus sodium azide (0.02% w/v ) as a

preservative. Stable for > 2 years at 4°C.

Bottle 2: NADH plus ATP, PEP and PVP.

Stable for > 2 years at either 4°C or -20°C.

Bottle 3: Acetate kinase plus pyruvate kinase and D-lactate

dehydrogenase suspension, 4.1 mL.

Stable for > 2 years at 4°C.

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