乙酸[乙酸激酶法]检测试剂盒 Acetic Acid (Acetate Kinase Manual Format) Assay Kit Megazyme K-ACETRM 现货促销

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货号: K-ACETRM
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 乙酸[乙酸激酶法]检测试剂盒  Acetic Acid (Acetate Kinase Manual Format) Assay Kit  Megazyme  K-ACETRM   现货促销
产品货号:K-ACETRM 

品牌:Megazyme  

订购热线: 400-811-9081

产品品名:乙酸[乙酸激酶法]检测试剂盒

英文品名:Acetic Acid (Acetate Kinase Manual Format) Assay Kit

规格型号:72 assays (manual) / 720 assays (microplate)

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

INTRODUCTION:
Given its importance and ubiquitous occurrence in foods, beverages and other materials, acetic acid ranks amongst the most measured analytes. However, unlike some other common analytes, traditional enzymatic methods for this acid present significant limitations. For instance, manual acetyl-coenzyme A synthetase (ACS) based formats are typified by unstable reagents during use, slow reaction times,multiple cuvette additions / absorbance readings and complex
calculations. While the advanced acetic acid kit (K-ACET) recently developed by Megazyme overcomes the key stability issue relating to the reconstitution of ACS for manual use, the overall format of the assay is still relatively inconvenient, requiring multiple cuvette additions and time-consuming data recording / processing. Thus Megazyme developed the current kit (K-ACETRM) based on acetate kinase (AK) and phosphotransacetylase (PTA) specifically to
overcome these limitations experienced by the analyst employing manual assay procedures. Unlike ACS based kits, this product is rapid (approx. 4 min), does not present reagent stability issues,is simple to perform, has extended linearity and gives a change in absorbance stoichiometric with the concentration of acetic acid (i.e. no complex equations are required and the raw absorbance data is processed like most other enzymatic kits). In common with other Megazyme acetic acid kits, K-ACETRM also benefits from > 2 years stability during use, as the key enzyme components are supplied as ready-to-use ammonium sulphate suspensions. Polyvinylpyrrolidone (PVP) has also been incorporated into the assay system to prevent interference from particular tannins found in wines (especially red
wines). As the pH (7.4) of the buffer system employed is significantly lower than in traditional ACS based kits (8.4), interference from spontaneous ester hydrolysis (causing creep reactions) is also minimised.

KITS:
Kits suitable for performing 72 assays in manual format (or 720 assays in microplate format) are available from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (24 mL, pH 7.4) and sodium azide (0.02% w/v) as a preservative.Stable for > 2 years at 4°C.
Bottle 2: Tablets (18) containing NADH plus ATP, PEP and PVP. Stable for > 2 years at -20°C.
Bottle 3: (x2) CoA, lyophilised powder.Stable for > 2 years at -20°C.
Bottle 4: D-Lactate dehydrogenase, phosphotransacetylase and pyruvate kinase suspension (1.5 mL).Stable for >2 years at 4°C.
Bottle 5: Acetate kinase suspension (1.5 mL). Stable for > 2 years at 4°C.
Bottle 6: Acetic acid standard solution (5 mL, 0.10 mg/mL).Stable for > 2 years at 4°C.

EQUIPMENT (RECOMMENDED):
1. Glass test tubes (round bottomed; 16 x 100 mm).
2. Disposable plastic cuvettes (1 cm light path, 3.0 mL).
3. Micro-pipettors, e.g. Gilson Pipetman (20 μL and 100 μL).
4. Positive displacement pipettor, e.g. Eppendorf Multipette?
- with 5.0 mL Combitip [to dispense 0.5 mL aliquots of
NADH/ATP/PEP/PVP/buffer (solution 2)].
- with 25 mL Combitip (to dispense 2.0 mL aliquots of
distilled water).
5. Analytical balance.
6. Spectrophotometer set at 340 nm.
7. Vortex mixer (e.g. IKA Yellowline Test Tube Shaker TTS2).
8. Stop clock.
9. Whatman No.1 (9 cm) and GF/A glass fibre filter papers.


乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31

L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49

 

 

 



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