供货周期: | 一周 |
品牌: | 子起生物 |
规格: | 咨询客服 |
货号: | K-AGLUA |
CAS号: | 咨询客服 |
α-葡萄糖醛酸酶检测试剂盒α-D-Glucuronidase assay Kit K-AGLUA Megazyme 现货促销
产品货号:K-AGLUA 品牌:Megazyme
订购热线: 400-811-9081 产品品名:α-葡萄糖醛酸酶检测试剂盒
英文品名:α-D-Glucuronidase assay Kit 规格型号:50 Assays per kit
A simple, reliable and accurate method for the measurement of alpha-D-glucuronidase in various enzyme preparations. Containing and aldouronic acid substrate and an α-D-glucuronidase control.
INTRODUCTION:
After cellulose, xylan is the second most abundant polysaccharide
found in nature. Xylan is a hemicellulose comprising a β-(1,4)-
linked xylan back bone substituted with various side chains such as
acetyl groups, α-(1-2/3)-linked L-arabinofuranose and α-(1-2)-linked
4-O-methylglucuronic acid. A repertoire of enzymes are required to
effect the complete degradation of plant cell wall polysaccharides in
the utilisation of plant biomass. Hence, complete degradation of xylan
requires the concerted action of various enzymes including endo-β-
(1,4)-xylanases, β-(1,4)-xylosidases, acetylxylan esterases,
α-L-arabinofuranosidases and α-glucuronidases.
The importance of carbohydrate degrading enzymes has increased
over recent years due to the growing focus of obtaining renewable
energy from plant biomass in the form of biofuels such as cellulosic
and hemicellulosic ethanol. Various research programmes are striving
to develop optimised enzyme mixtures or modified microorganisms
to increase the efficiency of biomass conversion and, as such, it is
important that the various enzyme activities can be easily measured.
Simple methods of measuring enzyme activity for some of the well
studied carbohydrate degrading enzymes such as endo-β-(1,4)-
cellulases, endo-β-(1,4)-xylanases, β-(1,4)-xylosidases exist. However
for some enzymes such as α-glucuronidases, current available methods
are cumbersome.
This kit (K-AGLUA) provides a simple method suitable for the specific
measurement of α-D-glucuronidase activity (α-D-glucosiduronate
glucuronohydrolase; EC 3.2.1.139 and xylan α-1,2-glucuronosidase;
EC 3.2.1.131).
KITS:
Kits suitable for performing 50 assays in manual format (or 200 assays
in microplate format) are available from Megazyme. The kits contain
the full assay method plus:
Bottle 1: Aldouronic acid mixture (tri:tetra:penta) (6 mL;
10 mg/mL).
Stable for > 5 years at -20°C.
Bottle 2: α-D-Glucuronidase solution (2 mL; ~ 1.0 U/mL, see
label on bottle).
Stable for > 2 years at -20°C.
Bottle 3: Stop Buffer (28 mL, pH 10) plus sodium azide
(0.02 % w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 4: NAD+ freeze dried powder.
Stable for > 5 years at -20°C.
Bottle 5: Uronate dehydrogenase suspension (1.1 mL).
Stable for > 2 years at 4°C.
乙醛检测试剂盒K-ACHYD13
乙酸 (ACS;分析仪格式)检测试剂盒K-ACETAF13
乙酸(ACS;手工格式)检测试剂盒K-ACET14
乙酸(AK;分析仪格式)检测试剂盒K-ACETAK14
乙酸(RM;快速手工格式)检测试剂盒K-ACETRM15
α-淀粉酶(“Ceralpha”方法)检测试剂盒K-CERA15
氨检测试剂盒K-AMIA16
氨(快速)检测试剂盒K-AMIAR16
直链淀粉 /支链淀粉检测试剂盒K-AMYL17
阿拉伯糖检测试剂盒K-ARAB17
L-阿拉伯糖/ D-半乳糖(快速)检测试剂盒K-ARGA18
阿拉伯木聚糖检测试剂盒K-ARAXYL18
L-精氨酸 /尿素 /氨(快速)检测试剂盒K-LARGE19
L-抗坏血酸检测试剂盒K-ASCO19
L-天冬酰胺酸/ L-谷氨酸酰胺/氨(快速)检测试剂盒K-ASNAM20
阿斯巴甜检测试剂盒K-ASPTM20
可用碳水化合物 /膳食纤维检测试剂盒K-ACHDF21
?-淀粉酶(“Betamyl”方法)检测试剂盒K-BETA21
β-(Mixedlinkage)检测试剂盒K-BGLU22
β-检测试剂盒K-YBGL22
β-葡聚糖酶(麦芽和微生物)检测试剂盒K-MBGL23
胆固醇检测试剂盒K-CHOL23
柠檬酸检测试剂盒K-CITR24
乙醇检测试剂盒K-ETOH24
蚁酸检测试剂盒K-FORM25
果糖(己糖激酶格式)检测试剂盒K-FRUCHK25
果糖(PAHBAH格式)检测试剂盒K-FRUC26
D-果糖 / D-葡萄糖检测试剂盒K-FRUGL26
D-果糖 / D-葡萄糖 (MegaQuant格式)检测试剂盒K-FRGLMQ27
半乳甘露聚糖检测试剂盒K-GALM27
葡甘露聚糖检测试剂盒K-GLUM28
D-葡萄糖酸 / D-葡萄糖酸内酯检测试剂盒K-GATE28
D-葡糖胺检测试剂盒K-GAME29
D-葡萄糖 (GOPOD格式)检测试剂盒K-GLUC29
D-葡萄糖(己糖激酶格式)检测试剂盒K-GLUHKR/L30
葡糖氧化酶检测试剂盒K-GLOX30
L-谷氨酰胺 /氨(快速)检测试剂盒K-GLNAM31
L-谷氨酸 (MSG)检测试剂盒K-GLUT31
甘油检测试剂盒K-GCROL32
D-3-羟丁酸检测试剂盒K-HDBA32
D-异柠檬酸检测试剂盒K-ISOC33
D-乳酸检测试剂盒K-DATE33
D-/L-乳酸检测试剂盒K-DLATE34
L-乳酸检测试剂盒K-LATE34
乳糖 / D-半乳糖(快速)检测试剂盒K-LACGAR35
乳糖/蔗糖 / D-半乳糖检测试剂盒K-LACSU35
乳果糖检测试剂盒K-LACTUL36
D-苹果酸检测试剂盒K-DMAL36
L-苹果酸检测试剂盒K-LMALR/L37
L-苹果酸 (Analyser格式)检测试剂盒K-LMALAF37
L-苹果酸(MegaQuant格式)检测试剂盒K-LMALMQ38
麦芽糖/蔗糖/ D-葡萄糖检测试剂盒K-MASUG38
D-甘露醇 / L-阿拉伯糖醇检测试剂盒K-MANOL39
D-甘露糖 / D-果糖 / D-葡萄糖检测试剂盒K-MANGL39
果胶鉴定检测试剂盒K-PECID40
植酸检测试剂盒K-PHYT40
Primary氨基氮 (NOPA)检测试剂盒K-PANOPA41
丙酮酸检测试剂盒K-PYRUV41
棉籽糖/ D-半乳糖检测试剂盒K-RAFGA42
棉籽糖 /蔗糖 / D-葡萄糖检测试剂盒K-RAFGL42
抗性淀粉检测试剂盒K-RSTAR43
D-山梨醇 /木糖醇检测试剂盒K-SORB43
破损淀粉检测试剂盒K-SDAM44
琥珀酸检测试剂盒K-SUCC44
蔗糖 / D-果糖 / D-葡萄糖检测试剂盒K-SUFRG45
蔗糖 / D-葡萄糖 (GOPOD格式)检测试剂盒K-SUCGL45
总膳食纤维检测试剂盒K-TDFR46
总淀粉 (GOPOD格式)检测试剂盒K-TSTA46
总淀粉(己糖激酶格式)检测试剂盒K-TSTAHK47
海藻糖检测试剂盒K-TREH47
尿素/氨(快速)检测试剂盒K-URAMR48
木聚糖酶 (Azo-Wax格式)检测试剂盒K-AZOWAX48
木聚糖酶(木聚糖酶 AX格式)检测试剂盒K-XYLS49
D-木糖检测试剂盒K-XYLOSE49
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