人封闭抗体(APLA)测定试剂盒

2015-09-07 19:45  下载量:0

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The kit assay Human APLA level in the sample,use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add APLA to wells, Combined antigen which With HRP labeled,become antigen – antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human APLA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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