资料摘要
资料下载Mouse osteonectin(ON) FOR RESEARCH USE ONLY Assay range:2.8ng/ml - 95ng/ml 96 determinations Purpose This kit allows for the determination of ON concentrations in Mouse Hippocampal tissue and other biological fluids. Principle of the assay The kit assay Mouse ON level in the sample, use Purified Mouse ON antibody to coat microtiter plate wells, make solid-phase antibody, then add ON to wells, Combined ON antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse ON in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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