资料摘要
资料下载Mouse GSK-3β FOR RESEARCH USE ONLY Assay range：6pmol/L - 200pmol/L 96 determinations Purpose This kit allows for the determination of GSK-3β concentrations in Mouse Hippocampal tissue and other biological fluids. Principle of the assay The kit assay Mouse GSK-3β level in the sample, use Purified Mouse GSK-3β antibody to coat microtiter plate wells, make solid-phase antibody, then add GSK-3β to wells, Combined GSK-3β antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse GSK-3β in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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