Monkey Col-IV ELISA kit

2015-10-14 11:24 下载量：2

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Monkey Col-IV FOR RESEARCH USE ONLY Assay range：1.6μg/L -60μg/L 96 determinations Purpose This kit allows for the determination of Col-IV concentrations in Monkey serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Monkey Col-IV level in the sample，use Purified Monkey Col-IV antibody to coat microtiter plate wells, make solid-phase antibody, then add Col-IV to wells, Combined Col-IV antibody which With HRP labeled,become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Monkey Col-IV in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海钰博生物科技有限公司

金牌会员 | 第10年

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