Monkey Col-ⅡELISA kit

2015-10-14 11:26 下载量：1

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Monkey Col-Ⅱ FOR RESEARCH USE ONLY Assay range：5μg/L -160μg/L 96 determinations Purpose This kit allows for the determination of Col-Ⅱ concentrations in Monkey serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Monkey Col-Ⅱ level in the sample，use Purified Monkey Col-Ⅱ antibody to coat microtiter plate wells, make solid-phase antibody, then add Col-Ⅱ to wells, Combined Col-Ⅱ antibody which With HRP labeled,become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Monkey Col-Ⅱ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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上海钰博生物科技有限公司

金牌会员 | 第10年

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