转录因子蛋白SIM1抗体

英文名称  Anti-SIM1 

中文名称  转录因子蛋白SIM1抗体 

转录因子蛋白SIM1抗体别    名  bHLHe14; Class E basic helix-loop-helix protein 14; SIM1; SIM1_HUMAN; Single minded homolog 1; Single-minded homolog 1. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg   

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish

产品类型  一抗    

研究领域  细胞生物 神经生物学 信号转导 转录调节因子  

蛋白分子量  predicted molecular weight: 85kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human SIM1 (2-80aa) 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

转录因子蛋白SIM1抗体产品介绍 The Per-Arnt-Sim (PAS) domain was identified as a 270 amino acid motif that mediates associations between various PAS family transcription factors. Several PAS domain family members have been identified including AhR, Arnt 1, and single-minded proteins (SIM1 and SIM2). The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator protein 1 (Arnt 1) is required for ligand-dependent nuclear translocation of the Ah receptor and is also necessary for Ah receptor binding to the XRE element. Both SIM1 and SIM2 inhibit AhR/Arnt dimerization, thus inhibiting transcriptional activation. The SIM genes are thought to be involved in the directing and regionalization of tissues during development and the SIM2 gene, which is located on chromosome 21, is a candidate for the gene responsible for Down syndrome.

Function : Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.

Subunit : Efficient DNA binding requires dimerization with another bHLH protein. 转录因子蛋白SIM1抗体Heterodimer of SIM1 and ARNT.

Subcellular Location : Nucleus.

Similarity : Contains 1 basic helix-loop-helix (bHLH) domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Contains 1 Single-minded C-terminal domain.

Database links : UniProtKB/Swiss-Prot: P81133.2