骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体elisa实验

骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体英文名称  Anti-BMP1 

中文名称  骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体 

别    名  BMP 1; BMP-1; Bone morphogenetic protein 1; Mammalian tolloid protein; mTld; PCOLC; PCP; ProCollagen C endopeptidase; Procollagen C proteinase; TLD; BMP1_HUMAN. 

浓    度  1mg/1ml 

规 格  0.2ml/200μg    

骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit  

产品类型  一抗    

研究领域  细胞生物 免疫学 信号转导 细胞骨架 细胞外基质  

蛋白分子量  predicted molecular weight: 98kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human BMP1 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.

骨形态发生蛋白1/胶原C蛋白肽链内切酶抗体Function : Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD).

Tissue Specificity : Ubiquitous.

Similarity : Belongs to the peptidase M12A family. Contains 5 CUB domains. Contains 2 EGF-like domains.

Database links : UniProtKB/Swiss-Prot: P13497.2